116 JOURNAL OF THE SOCIETY OF COSMETIC CHEMISTS of the replication technique. Several types of trichomes are distinguishable on the coleus leaf. Globular structures, such as those visible in Figures 2 through 5, were always observed in replicas taken from the forearm or thigh regions. Figure 7 illustrates that the globular structures are not present in replicas made of the palm of the hand, a woven fabric, and leather. The globular features observed in forearm and thigh regions are due to tran- spiratory processes at the surface of the skin. This information is important, because these globular features are often interpreted as artifacts of the replication process. This observation is further substantiated by Figure 6, which shows transpiratory processes occurring at the surface of leaves. The applicability of the replication method to the determination of the fate of an experimental polymer contained in a facial lotion is demonstrated in Figures 8 and 9. Figure 8 illustrates the topographical features of three areas of the forehead before 881250 10KV X50.0 688um Figure 7. Replication of (a and b) the palm and the hand (c) leather (d) woven material.

REPLICATION OF SKIN 117 •0295.S 10KY X500 60um SAMPLE'#?9-'': FORE Figure 8. Topographical features of three areas of the forehead, before treatment with either facial toner or facial lotion. '$AMP•E• • "- •QJJP •l .#.5.9 TDk0mSn) ' ' ' _. ' '- c•_ .-, '•.. d , .-•-•. ,. ,..• •' •, t•••:•..%•g•Tk •- ._•,--'• , •(•:lhr• • "•' PgA'" ' :3h•)'- . S,MPkEJ: :'H Figure 9. Topographical features of three are• of the forehead after application of a facial lotion: (A), (C), and (E) ten minutes after application (B) at one hour (D) at three hours (F) at five hours after application. treatment with either the facial toner or facial lotion (a blank). The same areas of the forehead are shown in Figure 9, 10 minutes after application of the facial lotion (A, C, E) and at one hour (B), three hours (D), and five hours (F) after application of the facial lotion. A substantial amount of the polymer was found on the forehead at one hour after