7.58 JOURNAL OF THE SOCIETY OF COSMETIC CHEMISTS containing solutions. The addition of hexachlorophene, indicated by the bottom broken line, reduces the level of skin flora even fhrther to approxi- mately 75% below the original count after six hours. 80 70 '• 60 o .c_ 50 0 '3 40 20 I0 ' 0 i 2 3 4 5 Time in hours Figure 9.--Face Plate Counts--Commercial after shave lotions. SUMMARY In vitro and in vivo test methods have been presented to show the ability of perfumes in after-shave lotions to reduce or inhibit the growth of skin flora. No attempts have been made to delineate the action of individual per- fume components since previous investigators have defined the relative activities of many essential oils and aromatic chemicals.

EFFECTS OF AFTER-SHAVE LOTIONS ON SKIN I I.ORA 759 100 9O 8O 7O 6O 5O 40 3O 2O 10 Solutions- no perfume Commercial Lotions Soluhons- with xach I orophen e I I I I I 2 .'5 4 5 6 Time in hours Figure 10.--Face Plate Counts--Averages: Test solutions without perfume Com- mercial lotions Test solutions with hexachlorophene. The in vivo and in vitro procedures outlined here confirm each other and offer a choice of quick, convenient and reproducible methods for deter- mining the effects of perfume in alcoholic lotions. (Received June 22, 1964)