168 .JOURNAL OF THE SOCIETY OF COSMETIC CHEMISTS 1970 Scholarship Grants in Cosmetic Sciences The Board of Directors of the Society ot/Cosmetic Chemists has allo- cated f$5000 for 1970 scholarship grants to further education in cosmetic science. The deadline for grant applications is April 30, 1970. For details write: Dr. Winthrop E. Lange The Purdue Frederick Company 99-101 Saw Mill River Road • Yonkers, New York 10701 The following schools received grants in 1969: Columbia University College of Pharmaceutical Sciences, New York, N. Y., Dr. Joseph Kanig, Dean Massachusetts College of Pharmacy, Boston, Mass., Dr. Harold Silver- man, Professor of Pharmacy Roosevelt University, Chicago, Ill., Dr. Walter Guthman, Chairman, Dept. of Chemistry St. John's University College of Pharmacy, Jamaica, N. Y., Dr. John Sciarra, Director, Graduate Division St. Louis College of Pharmacy, St. Louis, Mo., Dr. Joseph E. Harberle, Director of Pharmaceutical Sciences West Virginia University School of Pharmacy, Morgan town, W. Va., Dr. Paul Zanowiak, Assoc. Professor of Pharmaceutics Purdue University, School of Pharmacy, Lafayette, Ind., Dr. Glen J. Sperandio, Head, Department of Clinical Pharmacy University of Pittsburgh, School of Pharmacy, Pittsburgh, Pa., Dr. Lawrence H. Block, Assistant Professor of Pharmacy

]. Soc. Cosmetic Chemi.sts, 21, 16[}-182 (Mar. 4, 1970) Comparison of Several Procedures for the Extraction of Proteins from Human Epidermis* CHRISTOPHER CARRUTHERS, Ph.D.* Synopsis--PROTEINS were extracted from human EPIDERMIS with LITHIUM BROMIDE, UREA, and dilute ALKALI. ANTISERA were prepared in rabbits against these different proteins and IM- MUNODIFFUSION in agar was employed to investigate the relationships between the types of protein extracted by the three reagents. Also, absorption of the different antisera with the various proteins prior to immunodiffusion was uscd for this purpose. These expcriments revcaled that TONOVmmN (lithium bromide-extractable protein), alkali-extractable proteins of tonofibrin, and the alkali- and urea-extractable proteins of human epidcrmis wcrc related immunochemically or that the procedures employed extracted similar proteins in different amounts. Although the alkali-derived proteins of tonofibrin and the alkali-extractable pro- reins of human epidermis had different isoelectric points, tbcsc proteins had more in common with each other than with the urea-extractable proteins. INTRODUCTION Since the time o[ the original investigations of Rudall on proteins o[ bee[ snout epidermis (1), proteins of various types have been isolated from epidermis with solutions o[ urea, sodium hydroxide, and lithium bromide (1-11). The relationship ot• these proteins to keratinization has not yet been definitely established. In this report a comparison by im- munochemical procedures of the proteins isolated from human epidermis by several different methods is described. * Supported in part by Grant No. CA 07236 from the National Cancer Institute, U.S.P.H.S. ? Department of Biochemistry Research, Roswell Park Memorial Institute, and the New York State Department of Health, Buffalo, N.Y. 14203. 169