SOAP-INDUCED WINTER XEROSIS 219 (24) M. L. Williams, "Lipids in Normal and Pathological Desquamation," in Advances in Lipid Research, P.M. Elias, Ed. (Academic Press, San Diego, 1991), Vol. 24, pp. 211-262. (25) D. Saint Leger, A.M. Francois, J. L. Leveque, T. L. Stoudemayer, G. L. Grove, and A.M. Klig- man, Age associated changes in stratum comeurn lipids and their relation to dryness, Dermatologica, 177, 159-164 (1988). (26) G. Pierard, What does dry skin mean?, Int. J. Dermatol., 26, 167-168 (1987). (27) G. Imokawa, S. Akasaki, M. Hattori, and N. Yoshizuka, Selective recovery of deranged water- holding properties by stratum comeurn lipids, Jo Invest. Dermatol., 187, 758-761 (1986). (28) G. Imokawa, S. Akaski. Y. Minematsu, and M. Kawai, Importance of intercellular lipids in water- retention properties of the stratum comeurn: Induction and recovery study of surfactant dry skin, Arch. Dermatol. Res., 281, 45-51 (1989). (29) D. Saint Leger, A.M. Francois, J. L. Leveque, J. J. Stoudemayer, A. M. Kligman, and G. Grove, Stratum comeurn lipids in winter xerosis, Dermatologica, 178, 151-155 (1989). (30) M. Fartasch, T. L. Diepgen, and O. P. Hornstein, Morphological changes of epidermal lipid layers of stratum corneum in sodium lauryl sulphate induced dry skin: A functional and ultrastructural study, J. Invest. Dermatol., 96, 617 (1991). (31) M. Denda, J. Hori, J. Koyama, S. Yoshiida, R. Nabba, M. Takahashi, I. Horij, and A. Yamamoto, Stratum comeurn sphingolipids and free amino acids in experimentally-induced scaly skin, Arch. Dermatol. Res., 284, 363-367 (1992). (32) A. W. Fulmer and G. J. Kramer, Stratum comeurn lipid abnormalities in surfactant-induced dry scaly skin, J. Invest. Dermatol., 86, 598-602 (1986). (33) E. K. Bolsits, G. E. Nole, and M. C. Cheney, The refined regression method,J. Cutan. Aging Cosmet. Dermatol., 1, 155-163 (1989). (34) P. W. Wertz and T. Downing, Ceramides of pig epidermis: Structure determination, J. Lipid. Res., 24, 1135-1139 (1983). (35) P. W. Wertz, M. C. Miethke, S. A. Long, J. S. Strauss, and D. T. Downing, The composition of ceramides from human stratum comeurn and comedones, J. Invest. Dermatol., 84, 410-412 (1985). (36) M. Ponec, A. Weehelm, J. Kempenaar, A.M. Mornmaas, and D. H. Nugteren, Lipid composition of cultured human keratinocytes in relation to their differentiation, J. Lipid. Res., 28, 949-961 (1988). (37) P. K. Smith, R. I. Krohn, G. T. Hermanson, A. K. Mallia, F. H. Gartner, M.D. Provenzano, E. K. Fujimoro, N.M. Geoeke, B.J. Olsen, and D.C. Klenk, Measurement of protein using bicinchoninic acid, Anal. Biochem., 150, 76-85 (1985). (38) U. K. Laemmli, Cleavage of structural proteins during the assembly of the head of bacteriophage T4, Nature., 227, 680-685 (1970). (39) M. Fartasch, I.D. Bassukas, and T. L. Diepgen, (1993) Structural relationship between epidermal lipid lamellae, lameliar bodies and desmosomes in human epidermis: An ultrastructural study, Br. J. Dermatol., 128, 1-9. (40) R. Ghadially, M. L. Williams, S. Y. E. Hou, and P. M. Elias, Membrane structural abnormalities in stratum comeurn of autosomal recessive ichthyoses, J. Invest. Dermatol., 99, 755-763 (1992). (41) T. Kitahara, H. Shinta, and G. Imokawa, Quantitative analysis of desmoglein i in the stratum comeurn from UVB exposed skin, J. Derm. Sci., 4, 121 (1992). (42) G. K. Menon, R. Ghadially, M. L. Williams, and P.M. Elias, Lameliar bodies as delivery systems of hydrolyric enzymes: Implications for normal and abnormal desquamation, Br. J. Dermatol., 126, 337-345 (1992). (43) I. A. King, M. J. Wood, and P. R. Fryer, Desmoglein II-derived glycopeptides in human epidermis, J. Invest. Dermatol., 92, 22-26 (1989). (44) D. Bommannan, R. P. Potts, and R. H. Guy, Examination of stratum corneum barrier function in vivo by infrared spectroscopy, J. Invest. Dermatol., 95, 403-408 (1990). (45) P. W. Wertz, D.C. Swartzendruber, K. C. Madison, and D. T. Downing, Composition and mor- phology of epidermal cyst lipids, J. Invest. Dermatol., 89, 419-425 (1987). (46) R. Ghadially, L. Halkier-Sorensen, and P. M. Elias, The effects of petrolatum on stratum corneum structure and function, J. Am. Acad. Dermatol., 26, 387-396 (1992). (47) P. M. Elias and G. K. Menon, "Structural and Lipid Biochemical Correlates of the Epidermal Per- meability Barrier," in Advances in Lipid Research, P.M. Elias, Ed. (Academic Press, San Diego, 1991), Vol 24, pp. 1-26.

220 JOURNAL OF THE SOCIETY OF COSMETIC CHEMISTS (48) (49) (50) (51) (52) (53) (54) (55) (56) (57) (58) (59) (60) B. Melnik, J. Hollman, U. Hoffman, M. S. Yuh, and G. Piewig, Lipid composition of outer stratum corneum and nails in atopic and control subjects, Arch. Dermatol. Res., 282, 549-551 (1990). G. Imokawa, A. Abe, K. Jin, Y. Higaki, M. Kawashima, and A. Hidano, Decreased level of ceramides in stratum corneum of atopic dermatitis: An etiologic factor in atopic dry skin, J. Invest. Dermatol., 96, 523-526 (1991). A. Yamamoto, S. Serizawa, M. Ito, and Y. Sato, Stratum corneum lipid abnormalities in atopic dermatitis, Arch. Dermatol. Res., 282, 219-223 (1991). S. E. Friberg and I. Kayali, Water evaporation rates from a model of stratum corneum lipids, J. Pharm. Sci., 78, 639-643 (1989). P. W. Wertz and D. T. Downing, Epidermal ceramide hydrolase, J. Invest. Dermatol., 94, 590 (1990). D. L. Bissett and J. F. McBride, "Use of the Domestic Pig as an Animal Model of Human Dry Skin," in Models in Dermatology, H. Maibach and P. Lowe, Eds. (Karger, Basel, 1985), Vol 1, pp. 159-168. B. D. McKersie, J. H. Crowe, and L. M. Crowe, Free fatty acid effects on leakage, phase properties and fusion of fully hydrated model membranes, Biochim. Biophys. Acta, 982, 156-160 (1989). G. K. Menon, K. R. Feingold, M. Mao-Qiang, M. Schaude, and P. M. Elias, Structural basis for the barrier abnormality following inhibition of HMG CoA reductase in murine epidermis, J. Invest. Dermatol., 98, 209-219 (1992). H. Kido, N. Fukusen, and N. Katunuma, Inhibition of chymase activity by long chain fatty acids, Arch. Biochem. Biophys., 230, 610-614 (1984). R. White and M. Walker, Thermotropic and lyotropic behaviour of epidermal lipid fraction, Blochem. Soc. Trans., 18, 881-882 (1990). J. L. Leveque, G. L. Grove, J. Rigal, P. Corcuff, A.M. Kligman, and D. Saint Leger, Biophysical characterization of dry facial skin, J. Soc. Cosmet. Chem., 82, 171-177 (1987). I. Horij, Y. Nakayama, M. Obata, and H. Tagami, Stratum corneum hydration and amino acid content in xerotic skin, Br. J. Dermatol., 121, 282-284 (1989). A.M. Kligman, "The Biology of the Stratum Corneum," in The Epidermis, W. Montagna and W. C. Lobitz, Eds. (Academic Press, New York, 1964), pp. 387-433.