ABSTRACTS 265 Key words: poly (MPC), contact angle, non- hydrophilic, non-lipophilic, hydrogel membrane, barrier function, troubled skin, transepidermal wa- ter loss, permeation, pseudo-stratum corneum A New Method for Optical Evaluation of Tex- ture Manabu Fuse, Koichi Shimmoto Fundamental Research Laboratory, KOSE Corpora- tion A goniophotometer has ordinarily been used to evaluate color and texture of subjects. However, this method gives vast effort and a long time to measure, since incident and reflective angles must be changed in many times. Moreover, since only one point of subject can be measured, it is actually impossible to obtain two-dimensional information which are needed to evaluate texture. To overcome these dis- advantages, the new and unique method to evaluate color and texture of subject simultaneously is pro- posed. By recording images with multiple optical filters and a CCD camera, reverse calculating of ob- tained strength of reflective light can realize to mea- sure spectral reflectance whole over the subject's sur- face simultaneously. A new device has been con- structed based on our theory and tested on foundation painted on a sphere. The device could measure spectral reflectance at over 16000 points on the subject's surface at once. Moreover, changing high compositions of power spectrum given in spe- cific wavelength using FFT could evaluate texture of subject. Clearly, the proposed method offers signifi- cant advantages over conventional methods. Key words: direct measurement, color, texture, spectral reflectance, optical filter, CCD camera equipment, methodology, fast Fourier transform, goniophotometric method, reverse calculation Determination of Estradiol and Ethinylestradiol in Milk Lotion and Cream Hiroshi Tokunaga, Younson Chung, Tadasi Uchino, Masanori Ando, National Institute of Health Sci- ences The detennination of estradiol and ethinylestradiol in cosmetic milk lotion and cream has already been established in the Standard Method of Analysis for Hygienic Chemists with Commentary, 1995. But the harmful reagents against the human body such as chloroform and dichloromethane are used in the analytical procedures. In order to elim- inate the harmful reagents and simultaneously protect the determination of estradiol and eth- inylestradiol from the interference of parabens in most cosmetics, we estimated a new and convenient method. The milk lotions or creams were condensed onto the water bath and then estradiol or ethinyl- estradiol in the residue was extracted with ethanol. The extract was analyzed by the high-performance liquid chromatography using the ODS column (TSKgel ODS8oTM, 4.6 x 150 mm), the mixture of water, acetonitrile and methanol (3:1:1) as a mobile phase and the fluorescence detector (excitation and emission wavelength: 290 and 310 nm). By the use of the analytical method above we made sure that estradiol and ethinylestradiol could be determined without interference of parabens as conservatives or other ingredients. Key words: estradiol, ethinylestradiol, determina- tion, milk lotion, cream, HPLC, analysis, ODS, parabens, fluorescence detector Study on the Affinity of "Fine Clustered Water" for the Skin and the Human Hair *• Shinji Tsusaki '2, Kenichi Kakino '2, Kinya Tak- agaki '2, Makoto Kaneko '2 Toshiharu Kuwahara '2, Toshimitsu Hattori '2, Kazuhiro Matsushita '3 Ayaaki Ishizaki '4, Tokiji Kawamura '5 Seven Seeds Co., Ltd., '2 Department of Medical Zoology, Saitama Medical School '3, Department of Food Sci- ence and Technology, Faculty of Agriculture, Ky- ushu University '4, Department of Applied Chem- istry, Faculty of Engineering, The University of To- kyo .5 Our most interested phenomena are how the cluster size of water influences the extent of hydration of the skin and the human hair. In this study, two water samples ("fine clustered water" prepared by Hattori's method and commercial purified water) were used. •70-NMR spectra of these water samples were examined. Line widths of the •70-NMR sig- nals were 60 and 113 Hz for "fine clustered water" and commercial purified water, respectively, imply- ing that the averaged cluster size of "fine clustered water" is smaller than that of commercial purified water. The amounts of water hydrated to the skin and the human hair were measured by gravimetric method and •H-NMR spectroscopy. The results suggest that "fine clustered water" brings about an increase in the extent of hydration of the skin and the human hair, compared with the case of commer- cial purified water. We may assume that the differ- ence in an averaged cluster size between two water samples affects the extent of the osmosis and the hydration. It may be expected that such character- istics of "fine clustered water" contribute to the bet- ter moisture effect of the skin and the human hair. Key words: hydradon, skin, human hair, "fine clus- tered water", commercial purified water, moisture effect, •70-NMR, 1H-NMR, gravimetric method, osmosis