246 JOURNAL OF COSMETIC SCIENCE Decrease in the Skin Transparency Induced by Protein Carbonylation in the Stratum Comeum lchiro lwai, Tomohiro Kuwahara, Tetsuji Hirao standard by using an ultrasonic bath. The centrifuged and filtrated methanol extracts were analyzed by GC on an InertCap 624 capillary column. The recoveries of DEG from toothpaste fortified at the levels of 0.1, 0.5 and 1.0%, SHISEIDO Life Science Research Center, 2-2-1, were 103.6, 99.9, 99.4%, respectively. The quantitation Hayabuchi, Tsuzuki-ku, Yokohama 224-8558, Japan limit of DEG in toothpaste was 0.05 %. The proposed It has been reported in recent years that the carbonyl modification of protein plays a part in various diseases. The existence of the protein carbonylation in the stratum comeum also came to be known in the last few years, but the effect on the properties of the stratum comeum including the skin appearance was not clarified. In this study, we examined the influence of protein carbonylation in the stratum comeum to provide helpful information for skin care products focusing on transparency of the stratum corneum. Firstly, we developed a method to assess the protein carbonylation level numerically by the image analysis of tape-stripped stratum corneum reacted with a fluorescent hydrazide. The level of stratum comeum carbonylation of the sun-exposed site (face) was higher than that of unexposed site (upper arm), and the surface method could be applied to other cosmetics on the market without any interference. By using this method, DEG was detected the presence in 2 out of IO toothpaste samples set to the hotel. This analytical method should be useful for quality control for manufacturers and importers of cosmetics. White Blood Cell Fractions, Oxidation Index and Level of Stress in Acne Patients and Healthy Volunteers Motohiko Takahashi*, Mika Tan*, Satoshi Kimura*, Hiroshi Ikeno** *R&D Laboratories, POLA Chemical Industries, INC., 27- 1, Takashimadai, Kanagawa-ku, Yokohama 221-0833, Japan **Ikeno Clinic, Dermatology & Dennatological Surgery, l-14-4-3F, Ginza, Chuo-ku, Tokyo 104-0061, part of the stratum comeum showed a higher level of Japan carbonylation compared with the deeper layer. Stratum corneum carbonylation was induced by in vitro UV irradiation. In addition, it was shown that the skin transparency score was significantly low in the woman with high level of protein carbonylation in the stratum comeum of the cheek. An experimentally carbonylated stratum corneum sheet in vitro became opaque. Among the amino acids investigated, L-lysine was the most effective to prevent carbonylation of the stratum comeum ex vivo. Moreover, L-lysine inhibited the decrease in the skin transparency caused by experimental carbonylation of the stratum comeum in the human skin. These results suggest that preventing the carbonylation of the stratum corneum due to adverse effects from the environment by I-lysine could improve the skin transparency, Determination of Diethylene Glycol in Toothpaste Mitsuko Kamimura, Kumiko Mizuno, Koichi Kuromi, Chie Ueno, Hiroyuki Kurita, Kazuhiro Owada, Masatoshi Yamamoto Department of Drug and Food Science, Shizuoka Institute of Environment and Hygiene, 4-27-2, Kita-Ando, Aoi-ku, Shizuoka 420-8637, Japan A rapid and simple method using gas chromatography (GC) for the determination of diethylene glycol (DEG) in toothpaste was developed. DEG in toothpaste was extracted with methanol containing 1,4-butanediol as the internal In recent years, a lot of people have suffered physical and mental stress. It makes sympathetic nerves dominant, and autonomic nerves unbalanced. Disruption of the balance in autonomic nerves leads to the increase of granulocyte, and causes various undesirable physical conditions. We investigated the relationship between stress and acne in order to confirm the influence of stress on skin conditions. The degree of subjective stress, and the ratio of neutrophils or lymphocytes in leukocytes were measured in acne patients and healthy volunteers. As it is suggested that the increase of the neutrophil ratio by stress makes reactive oxygen, and raises the degree of oxidation in the blood, we also measured the antioxidant action and the degree of oxidative stress by FRAS4. Additionally, the lifestyle characteristics in each group was investigated in order to confirm the relationship with stress by a questionnaire survey. According to the results of the questionnaire survey, acne patients find themselves readily suffering from acne anytime. Compared with healthy volunteers, acne patients have more undesirable lifestyles and higher stress degrees than healthy volunteers. Further, the Biological Antioxidant Potential (BAP), an antioxidant index measured in acne patients, was significantly lower (p0.0001) than that of healthy volunteers. The acne patients had a higher ratio of neutrophils and a lower ratio of lymphocytes compared to the healthy volunteers. Among the acne patients, the neutrophil ratio was higher in those with high stress (p0.05). These results suggest that acne patients easily feel

ABSTRACTS 247 higher stress against various loads, and the stress leads to becomes a high-speed thin-film spin flow. This flow the increase in the neutrophil ratio and the oxidation possibly allows the peripheral speed to be over 30 mis, degree, which exacerbates the acne lesions. which was unable to be obtained by a conventional high- A High-speed Mixer Enabling Particle Design Akihito Shundo Emulsification & Dispersion Technology Laboratory, PRIMIX Corporation, 8-16-43, Ebie, Fukushima-ku, Osaka 553-0001, Japan When fluid is mixed by a rotor and small vessel that has a narrow clearance between its wall and the rotor with the upper part covered by an endplate, the processing fluid speed mixer. As a result, the processing fluid obtains more and homogenous energy. In an actual experiment, we compared a high-speed thin-film spin mixer with a conventional high-speed mixer upon an emulsification experiment with soybean oil. The experiment proved that the former mixer could control the particle size distribution. It also proved that the same result could be obtained by continuous operation as by batch operation. In conclusion, the high-speed thin-film spin mixer can control particle size distribution with a high efficiency.