Title Formulation type Drug Evaluation Refs. CoQ10and retinaldehyde co-loaded nanostructured lipid carriers for effi cacy evaluation in wrinkles Gel containing nanostructured lipid carriers (NLCs) loaded with CoQ10 and retinaldehyde CoQ10and Retinaldehyde In vitro physicochemical characterization: (141) Examining the NLCscolloidal d system morphology was performed by SEMe Evaluating both the free gel and the NLCs containing gel spreadability and rheological behavior were determined by the cone-and-plate viscometer HPLC-based stability study A comparative drug release study was performed from NLCs and NLCs-gel In vitro cell culture studies were carried out for both cellular uptake and viability Ex vivo studies were carried out for assessment of skin permeation, distribution and dermal pharmacokinetics In vivo study was conducted by using animal model The skin irritation test on Sprague-Dawley rats was performed by using a vapometer to measure trans epidermal water loss Evaluating the therapeutic effi cacy of the optimized formulations on female Swiss albino mice after wrinkles induction Supplementary table I Continued JOURNAL OF COSMETIC SCIENCE 344

Title Formulation type Drug Evaluation Refs. Development of herbal cosmetic cream with Curcuma longa extract–loaded transfersomes for antiwrinkle effect Transfersomal cream Curcumin from Curcuma longa extract In vitro characterization of curcumin containing transfersomes: (104)) Morphological examination of the vesicles using transmission electron microscopy (TEMf) Spectrophotometric determination of the entrapment effi ciency Particle size and zeta potential measurement by a Malvern Zetasizer Storage stability over 6 months In vitro skin permeation and deposition studies were carried out by using 2-stage modifi ed Franz diffusion cells. Characterization of curcumin incorporated transfersomal cream Physicochemical evaluation of the cream (color, odor, content, pH, acid, ash, and saponifi cation value) The irritation test on human volunteers was carried out with determination of the erythemal score established by the Indian standards In vivo studies were carried out on female human volunteers to evaluate the skin elasticity using a cutometer Topical formulation containing NGN: effi cacy against ultraviolet B irradiation-induced skin infl ammation and oxidative stress in mice Conventional topical formulations NGN In vitro evaluation of NGN antioxidant activity was performed by using FRAPg, ABTS, h hydroxyl, iron-dependent and iron independent lipid peroxidation tests (50) Physicochemical characterization of the developed topical formulations was detected In vivo evaluation of the effi cacy of the NGN containing topical formulations in sex matched hairless mice subjected to UVBi radiation of measured intensity was performed by using various measurements (skin edema, cytokine measurement, FRAP, ABTS, catalase assay, lipid peroxidation, superoxide anion production, glutathione assay and others) Supplementary table I Continued SKIN-AGING AND INFLAMMAGING TREATMENT 345