SYSTEMIC EFFECT OF TOPICALLY APPLIED COSMETICS ß 289 Ef[ect of Application• of I-Iot, rnonem •50C •00C 5O0 1 2 $ 1 • 5 • 5 6 7 1 • 5 ß 5 6 7 $ week inter, val• Figure 3.--The increase weight of hair after estradiol treatment, and the decrease of the weight after testosterone therapy is evident in all three groups. The reversibility o f this phenomenon is obvious after changing from estradiol to testosterone and vice versa. an adrenal disfunction, we saw su•cient histological evidence on eight animals, which indicated a functional disorganization. The most re- markable changes consisted in the derangement of the fascicular zone (Fig. 5), increased mirotic activity in the inter]ocking zone, especial]y ' . ..:•.. -'L•e.'.• •"•. '" ' . •"• ' ". '• t•.:•:::.•':'•.. -" --' '• '•'-'" •:' ' •-'•.:' "%• '•- "•.- ' ':• .... i- .• •:•'- -•, ' •,x. r.•'"-'% •5 ..' • '4½.-z.. '- •'¾ . •.: . -- "2:7..•': ' . .• : • . • • •-• - .'•' • .... " -•. • .... •' • ß •-• . • .... ' :: ..... .•, • .. • •-• .. ,0'-• ,?•, .--.a :,•-%•__ :-, -• :...•-. •, ....... • .,• ... .•, ._. ,_ ,.• .• ,•.• ..... . • • *•, '{½-•'.•. ., ,.:-• - .. .. .... • .. _ • ,. • '• . • • .• ,. . • • •. , • •., ,.• • .-, •'*'E• '•- .. . •.. •.%•[,• •'• - .. . ,'. . .• .,***- ...- , . - :: ,., --. :• .. •.• =..•.- **•. : -. . ... •'.*• : . r. ""'- ' '" Figure 4.--Section of the skin of a castrated male rabbit. Note the reduced size of sebaceous glands and the several active hair follicles.

290 JOURNAL OF THE SOCIETY OF COSMETIC CHEMISTS .-•.' :::•"'•.. '•-..::•'.".'-..••'.:"Z.• • .,z •-' • . •' •r'" •.-• '• • . • - • '••,•?'•e•'.• •' .• •. . •.: ' .::•: --• • .•-'•.•.-• •.. :.•?.%.•:'•..•'-•_•.. ::•--• ..... • ...:..:• • ... Figure 5.•Section of cortex of adrenal of a testosterone castrated rabbit. Note the derangement of the glomemlar zone and a few lipid deposits in vacualized cells. Figure 6.--Section of the adrenal of an estradiol treated castrated male rabbit. Note the derangement of the glomerular zone with many large vacuolized cells.