EFFECTS OF AFTER-SHAVE LOTIONS ON SKIN FLORA 749 Approximately 20 ml. of the seeded agar was poured into sterile Petri dishes 100 X 20 min. and allowed to harden. After the agar had solidified, two openings were made in each plate, using a sterile cork borer. These were 18 min. in diameter and approximately 20 min. from the edge of the plates. Exactly 0.2 ml. of the material to be tested was placed in the openings. Figure 1 shows a control plate inoculated with ztspergillus niger Figure 1.--Control plate showing no zone of inhibition. Figure 2.--Aspergillus niger culture showing about 3 mm. zone of inhibition. showing no zone of inhibition. The bacterial plates were incubated for forty-eight hours at 37øC and the fungal culture for five days at room temperature before measuring the zones of inhibition. Typical zones of inhibition are shown in Figs. 2-4. Figure 2 is a culture of •tspergillus niger with a zone of inhibition of approximately 3 ram. and was obtained by using solution •7, containing the after-shave lotion ingre- dients and 3% perfume. Figure 3 shows a culture of Penici//ium with a

750 JOURNAL OF THE SOCIETY OF COSMETIC CHEMISTS Figure 3.--Penicillium culture showing large zone of inhibition. Figure 4.--Candida albicans culture showing typical zone of inhibition. TABLE II ZONES OF INHIBITION (MM.) Staphylo- Atsper- Face coccus gillus Bacteria .4ureus Niger Penicillium Candida Sp. Atlbicans Solution #1: water and ethyl alcohol 0 Solution #2: water, ethyl alcohol and benzyl alcohol 0 Solution #3: water, ethyl alcohol and propylene glycol 0 Solution #4: water, ethyl alcohol, benzyl alcohol and P. G. Solution #5: water, ethyl alcohol, benzyl alcohol, P. G. and 0.75% perfume 7 Solution #6: water, ethyl alcohol, bcnzyl alcohol, P.G. and 1. perfume 7 Solution #7: water, ethyl alcohol, benzyl alcohol, P. G. and 3. perfume 8 o o o o o o o o o o o o o o o o