70[ Average Scurf Weights of Ten Subjects 'ø I 50 i.-- //• 40L ,"/•//• i ',,"'' /'-., • /// '\•,' ',/ ",, ,,, I I/ \1 \ • I \\L•ft •o• • \^ ,, ,,,/,,,.., ,,, I .,' V x., •"' '•/ /x, ,o[ ,I I, I. 0 I0 ZO 30 0 50 60 70 80 Days Figure 1. Average scurf weights in mg of aU subjects The Average Percentage Variation in Scurf Weights During Each of the Treatment Periods from those of the Pre-Treatment Period Treatment Treatment Treatment Treatment Subject Period A Period B Period C Period D Left Side 1 +13.4 -23.5 -58.5 2 +5.7 +7.7 -26.6 3 -7.1 -26.2 -52.9 4 -5.9 -30.6 -28.2 5 +53.1 +95.3 +48.2 6 +24.8 --36.8 --50.0 7 +17.3 --16.3 --8.9 8 +16.5 +7.6 +5.0 9 +41.9 --34.1 --62.2 10 -- 26.0 -- 47.8 -- 46.0 Ave. +9.1 --16.8 --33.2 Right Side 1 --69.7 -84.4 -85.2 2 --23.3 --38.7 -54.1 3 -51.1 -77.6 -76.9 4 +1.0 --37.9 -26.7 5 +4.2 +2.1 -25.4 6 --25.8 -68.6 -64.8 7 --13.8 -27.2 -34.1 8 +24.4 +0.8 -1.6 9 +11.1 -35.8 -70.0 10 -49.9 -70.9 -65.2 Ave. -- 30.0 - 52.6 - 55.4 -42.5 -26.0 -60.6 ß ß .a +89.0 --51.9 --30.9 --10.4 --65.3 -58.5 -28.4 --89.3 -46.7 --75.4 ß . .a --53 -77 2 -50 4 -12 4 --61 8 --76 7 --56 4 Not done.

FLORA OF THE SCALP AND DANDRUFF PRODUCTION I •0000 ILOOOO 90000 70000 50000 30000 I0000 0 Plate Counts per mg. of Scurf Samples for Ten Subjects I II •x • I I • I :•0 30 40 5O 60 Doys Left• I I Right I I I I •o Figure 2. Average bacterial plate counts per mg of scurf sample for all subjects 145,000/mg. The counts on the left were much higher than on the right. During Period D, with the cessation of anti-microbial treatment and the application of a suspension of mixed bacteria, the counts quickly rose on both sides, averaging 200,000/mg. The average P. ovale count during the pre-treatment period was the same for both sides (17 per immersion oil objective fidd). During Period A there was a decrease in P. ovale counts on the right side in nine subjects. The left side counts of all subjects showed an increase during this time. The average count was 13 per field on the right and 47 per fidd on the left. Although the actual reduction in P. ovale counts on the right side might not seem to be significant, it is possible that some or- ganisms were picked up by the HairVac as a carry-over from the left. A microscopical comparison of scurf from both sides was made. Prepara- tions from the left side showed a greater number of organisms embedded in the scurf particles than from the right side. During Period B when both sides were treated with nystatin the average number of P. ovale cdls in nine subjects was seven per field for both sides. One subject had high counts throughout the entire experi- ment. Periods C and D showed very high counts, as might be expected in the absence of any nystatin application. Despite continuous nystatin treatment on the right side for most of the experiment it was very un- usual to find a scurf sample completely negative for P. ovale.