714 JOURNAL OF THE SOCIETY OF COSMETIC CHEMISTS displayed a hyperplastic bulbar epithelium and were located much deeper in the dermis than normal. Glinically, hair had not regrown in the treated areas. The guinea pig skin was also relatively nonreactive, with the principal lesion being slight (or slight to moderate) acanthosis. In the guinea pig skin taken 16 and 24 hours after treatment, this slight acanthosis had a zonal pattern suggestive of reactions along corneum scratches caused by clipper blade teeth. In the hairless mouse, applica- tions of oil ot5 bergamot resulted in a rather severe mononuclear, and later granulocytic, cell infiltration into the dermis after 24 hours. Despite the lack of gross lesions, the hamster showed marked derreal and moderate epidermal lesions after 24 hours. Early resolution of these changes were noted, however, in the 72-hour section. ACKNOWLEDGMENTS Assistance is acknowledged to Dr. Millard Maienthal for preparing pure bergapten, psoralen, and hydroxypsoralen to Mr. Ronald Yates for preparing pure limettin and 7-methoxy-5-geranoxycoumarin to Miss Susan Barkan for spectrophotofiuorometry to Mr. J. Jones and Mr. U. Gieri for analyzing certain bergapten samples and to Mr. Paige Yoder for technical assistance. Dr. John Epstein provided helpful suggestions in the conduct of the human studies. (Received April 30, 1970) REFERENCES (1) Tusing, T. 1V., Vernon, M. L., and Morrish, E. P., Biological evaluation of perftunes, Med. Ann. D.C., 32, 90-5 (1963). (2) Epstein, E., Perfnme dermatitis in men. J. Amer. Med. Ass., 209, 911-3 (1969). (3) Freund, E. /2ber bisher noch nicht beschriebene Kfinstlicke hautverfarbungen, Dermatol. Wochenschr., 63, 931-6 (1916). (4) Rosenthal, F., Berliner Dermatologische Gesselschaft, Dermatol. Zh., 42, 295 (1925) Der Berlockdennatitis, Dermatol. Wochenschr., 86, 242-3 (1928). (5) Kuske, H., Experimentelle unterschungen zur photosensibilisierung der haut durch pfianzliche wirkstaffe, Arch. Dermatol. Syphilol., 178, 112-23 (1938). (6) Ohme, C.,/2bet die Zusamensetzung des Bergamottols, Ann. Chem., 31, 316-21 (1839). (7) Pathak, M. A., Daniels, F., and Fitzpatrick, T. B., The presently known distribution of fnrocoumarins (psoralens) in plants, J. Invest. Dermatol., 39, 225-39 (1962). (8) Thekaekara, M.P., Kruger, R., and Duncan, C. H., Solar irradiance measurements from a research aircraft, Appl. Opt., 8, 1713-32 (1969). (9) Jagger, J., A small and inexpensive ultraviolet dose-rate meter useful in biological experiments, Radiat. Res., 14, 394403 (1961). (10) Burdick, K. H., Phototoxicity of Shalimar perfume, Arch. Dermatol., 93, 424-5 (1966). (l l) Klaber, R., Phyto-photo-dennatitis, Brit..L Dermatol. Syph., 54, 193-211 (1942).

PERFUME PHOTOTOXICITY 715 (12) Cieri, U. R., Characterization of the steam nonvolatile residue of bergamot oil and some other essential oils, ]. Ass. Oi]ic. Anal. Chem., 52, 719-28 (1969). (13) Lerner, A. B., Denton, C. H., and Fitzpatrick, T. B., Clinical and experimental studies with 8-methoxypsoralen in vitiligo, ]. Invest. Derrnatol., 20• 299-314 (1953). (14) Harber, L. C., Harris, H., Leider, M., and Baer, R. L., Berlock dermatitis: A technique for its deliberate reproduction, Arch. Dermatol., 90• 572-6 (1964). (15) Code of Federal Regulations, Title 21, Chapt. 1, Para, 191.6(e). (16) Caporale, G., Musajo, L., Rodighiero, G., and Boccichetti, F., Skin-photosentizing activity of some methylpsoralens, Experientia, 23, 985-6 (1967). (17) Pathak, M. A., Worden, L. R., and Kaufman, K. D., Effect of structural alterations on the photosensitizing potency of furocoumarins (psoralens) and related compounds, ]. Invest. Dermatol., 48, 103-18 (1967). (18) Musajo, L., Rodighiero, G., and Caporale, G., Activit• photodynamique des coumarins naturelies, Bull. Soc. Chim. Biol., 36, 1213-24 (1954). (19) Musajo, L., and Rodighiero, G., The skin-photosensitizing furocoumarins, Experientia, 18, 153-61 (1962). (20) Pathak, M. A., and Fitzpatrick, T. B., Relationship of molecular configuration to the activity of furocoumarins which increase the cutaneous response following long wave ultraviolet radiation, ]. Invest. Dermatol., 32, 255-62 (1959). (21) Guenther, Ernest, The Essential Oils, Vol. II, D. Van Nostrand, Princeton, N.J., 1949, p. 662.