DMDM HYDANTOIN AS A COSMETIC PRESERVATIVE Table III Henna Cream Conditioner Formula No. 10 11 12 13 14 Water 46.20 46.50 46.55 46.45 46.20 Corn Oil (BHT/BHA) 0.30 Same Same Same Same Octoxynol-9 1.00 Same Same Same Same Sodium Chloride 0.60 Same Same Same Same Dimethyl Amino Propyl Lanolin Plus Amide Dietho-Sulfate Quaternium 1.20 Same Same Same Same Stearalkonium Chloride 2.00 Same Same Same Same Cetyl Lactate 3.00 Same Same Same Same Stearamine Oxide 3.50 Same Same Same Same Stearamidopropyl Dimethyl Amine 3.50 Same Same Same Same Laureth-23 5.00 Same Same Same Same Lanolin 2.00 Same Same Same Same Stearyl & Fatty Alcohols 12.00 Same Same Same Same Hydrolyzed Animal Protein 18.10 Same Same Same Same Henna--Neutral 0.10 Same Same Same Same Acetamide MEA 1.00 Same Same Same Same Methylparaben -- 0.10 -- -- -- Propylparaben -- 0.10 -- -- -- Quaternium-15 -- - 0.15 -- -- DMDM Hydantoin -- -- -- 0.25 ,0.50 Total 99.50 100.00 100.00 100.00 100.00 Table IV Hand Cream Formulas Formula No. 15 16 17 18 19 Ingredients, % Water 74.16 73.96 74.01 73.96 73.76 Lanolin 0.90 Same Same Same Same Lanolin Alcohol 0.90 Same Same Same Same Stearyl + Fatty Alcohol 4.30 Same Same Same Same Stearic Acid 2.60 Same Same Same Same Silicone Oil 1.00 Same Same Same Same Butyl Stearate 10.00 Same Same Same Same Jojoba Oil 0.50 Same Same Same Same Carbomer 934 0.22 Same Same Same Same Propylene Glycol 2.00 Same Same Same Same Dioctyl Sodiumsulfosuccinate 0.16 Same Same Same Same Allantoin 0.10 Same Same Same Same PCA 0.10 Same Same Same Same Panthenol 0.10 Same Same Same Same Triethanol Amine 0.26 Same Same Same Same Hydrolyzed Animal Protein 2.70 Same Same Same Same Methylparaben -- 0.10 -- -- -- Propylparaben -- 0.10 -- -- -- Quaternium-15 - - o. 15 - - DMDM Hydantoin -- -- -- 0.20 0.40 Total 100.00 100.00 100.00 100.00 100.00

88 .JOURNAL OF THE SOCIETY OF COSMETIC CHEMISTS three other common preservatives, Methylparaben, Propylparaben and Quaternium-15, were included for comparison purposes. (All concentrations of DMDM Hydantoin referred to hereafter in this paper are of the 55% aqueous solution.) The nineteen samples were actually variations of the following four typical cosmetic products: (a) Lotion Shampoo (b) Liquid Hair Conditioner (c) Henna Cream Conditioner (18.1% animal protein) (d) Hand Cream Special procedures used for preparing the different formulations were as follows: Formula Preservative Code No. Product Used Formulation Procedure 1. Lotion Shampoo Methylparaben (0.1%) Propylparaben (0.03%) 2. Lotion Shampoo Quaternium-15 (0.3%) 3. Lotion Shampoo DMDM Hydantoin (0.3%) 4. Lotion Shampoo DMDM Hydantoin (0.15%) 5. Lotion Shampoo Methylparaben (0.1%) Propylparaben (0.1%) 6. Liquid Hair Methylparaben (0.1%) Conditioner Propylparaben (0.03%) 7. Liquid Hair Quaternium-15 (0.15%) Conditioner 8. Liquid Hair DMDM Hydantoin (0.15%) Conditioner 9. Liquid Hair DMDM Hydantoin (0.15%) Conditioner 10. Henna Cream Control Conditioner 11. Henna Cream Methylparaben (0.1%) Conditioner Propylparaben (0.1%) 12. Henna Cream Quaternium-15 (0.15%) Conditioner 13. Henna Cream DMDM Hydantoin (0.25%) Conditioner 14. Henna Cream DMDM Hydantoin (0.50%) Conditioner 15. Hand Cream Control 16. Hand Cream Methylparaben (0.1%) Propylparaben (0.1%) 17. Hand Cream Quaternium-15 (0.15%) 18. Hand Cream DMDM Hydantoin (0.2%) 19. Hand Cream DMDM Hydantoin (0.4%) Heated to 80øC. to solubilize the Parabens. Heated to 80øC. to solubilize the Quaternium-15. Heated to 80øC. Actually not necessary for DMDM Hydantoin. Mixed at room temperature. Heated to 80øC. to solubilize the Parabens. Heated to 80øC. to solubilize the Parabens. Heated to 80øC. to solubilize the Quaternium-15. Heated to 80øC. Actually not necessary for DMDM Hydantoin. Mixed at room temperature. Mixed at room temperature. Heated to 80øC. to solubilize the Parabens. Heated to 80øC. to solubilize the Quaternium-15. Mixed at room temperature. Mixed at room temperature. Mixed at room temperature. Heated to 80øC. to solubilze the Parabens. Heated to 80øC. to solubilize the Quaternium-15. Mixed at room temperature. Mixed at room temperature.