DMDM HYDANTOIN AS A COSMETIC PRESERVATIVE 93 Table IX Hand Cream All of the preservatives tested separately failed to protect the hand cream from all microorganisms. The Parabens (Sample//16) failed on yeast and bacteria growth. The Quaternium-15 (Sample//17) failed on yeast growth. The DMDM Hydantoin (Sample //18) failed on all microorganisms. However at higher use concentration (Sample//19), the DMDM Hydantoin did control all the bacteria microorganisms. Table IX Hand Cream Microorganism Count Per Gram of Product Organism and Initial Preservative System Count 7 Day 14 Day 21 Day 28 Day Twenty-eight Day Preservative Test //15 A.n. 1.4 x 10 6 2.2 x 10 4 3.0 x 10 4 2.8 X 10 4 2.6 x 10 4 C.a. 6.4 X 106 2.3 ( 106 1.4 X 107 1.0 x 107 1.8 X 107 None E.c. 3.4 x 106 1.2 x 106 1.4 x 108 2.0 x 108 2.8 x 108 S.a. 8.5 x 106 2.4 x 106 8.0x 10 ? 2.6 x 108 3.0 x 109 P.a. 4.0 x 106 3.0 x 106 5.5 x 107 6.0 x 107 7.0 x 108 //16 A.n. 1.4 x 106 100 100 0 0 Methylparaben C.a. 6.4 x 106 3.5 ( 106 7.7 ( 106 8.0 x 107 2.5 x 108 0.1% E.c. 3.4 x 106 5.0 x 106 1.7 x 107 2.4 x 107 4.1 x 108 Propylparaben S.a. 8.5 x 106 1.8 x 106 2.3 x 107 1.5 x 108 2.4 x 108 0.1% P.a. 4.0 x 10 6 9.0 x 10 6 1.2 x 10 7 3.1 x 10 8 4.5 x 10 8 //17 A.n. 1.4 x 106 100 0 0 0 C.a. 6.4 x 106 1.7 x 106 3.9 x 105 1.7 x 107 3.0 x 107 Quaternium-15 E.c. 3.4 x 106 100 0 0 0 0.15% S.a. 8.5 x 106 100 0 0 0 P.a. 4.0 x 106 100 0 0 0 //18 A.n. 1.4 x 106 Overgrown Overgrown Overgrown Overgrown Mold Mold Mold Mold C.a. 6.4 x 106 5.0 x 106 5.5 x 105 6.0 x 106 4.2 x 107 DMDM Hydantoin E.c. 3.4 x 106 8.5 x 106 9.0 x 105 2.0 x 106 2.6 x 106 0.2% S.a. 8.5 x 106 2.4 x 106 4.0 x 103 3.0 x 103 200 P.a. 4.0 x 106 7.0 x 106 2.0 x 106 4.0 x 106 5.0 x 106 //19 A.n. 1.4 x 106 Overgrown Overgrown Overgrown Overgrown Mold Mold Mold Mold C.a. 6.4 X 106 6.1 X 105 5.8 X 106 2.4 x 105 2.9 X 105 DMDM Hydantoin E.c. 3.4 x 106 100 0 0 0 0.4% S.a. 8.5 x 106 100 0 0 0 P.a. 4.0 x 106 100 0 0 0 (0) denotes no growth

94 JOURNAL OF THE SOCIETY OF COSMETIC CHEMISTS TOXICITY A tabulation of the toxicity results is shown in Tables X and XI Table X DMDM-Hydantoin-Toxicity Single Dose Acute Oral LD•o Female Rat (Sprague Dawley strain) Male Rat (Sprague Dawley strain) 50% Female, 50% Male Rats (Sprague Dawley strain) 3.8g/Kg body weight 2.7g/Kg body weight 3.7g/Kg body weight Single Dose Acute Dermal LD•o, g/kg Body Weight Rabbit, New Zealand white strain Product was rated not toxic derreally. 20 Acute Primary Derreal Irritation Rabbit, New Zealand strain 1.5 Primary Skin Irritation Index (Avg. 24 & 72 hour scores) Product was rated as not irritating to the skin. Acute Inhalation LC•o (Rat) Rat, albino, Sprague Dawley strain LC•o was in excess of 204.16 milligrams per liter of air. Product was rated not toxic by inhalation exposure. Aquatic Toxicity, 96 Hour TL•o DMDM Hydantoin Reference Pesticide p,p-DDT Rainbow Trout 514 ppm 0.025 ppm Bluegills 173 ppm 0.0108 ppm Daphnia Magna 37 ppm 0.0019 ppm Bird Wildlife 8-Day Dietary LC•o Mallard Ducks Bobwhite Quail Acute Oral LD•o Mallard Ducks 5000 ppm 5000 ppm 10g/kg Body Wt.