DMDM HYDANTOIN AS A COSMETIC PRESERVATIVE 91 Table VII Liquid Hair Conditioner All preservatives tested effectively controlled all microorganisms. Table VII Liquid Hair Conditioner Microorganism Count Per Gram of Product Organism and Initial Preservative System Count 7 Day 14 Day 21 Day 28 Day Twenty-eight Day Preservative Test //6 A.n. 1.4 x 106 100 0 0 0 C.a. 6.4 X 10 6 100 0 0 0 Methylparaben 0.1% E.c. 3.4 x 106 100 0 0 0 Propylparaben 0.03% S.a. 8.5 x 106 100 0 0 0 P.a. 4.0 x 106 100 0 0 0 //7 A.n. 1.4 X 10 6 100 0 0 0 C.a. 6.4 x 106 100 0 0 0 Quaternium-15 0.15% E.c. 3.4 X 10 6 100 0 0 0 S.a. 8.5 X 106 100 0 0 0 P.a. 4.0 x 106 100 0 0 0 //8 A.n. 1.4 x 106 100 0 0 0 C.a. 6.4 X 106 100 0 0 0 DMDM Hydantoin 0.15% E.c. 3.4 x 10 6 100 0 0 0 (80 ø) S.a. 8.5 x 106 100 0 0 0 P.a. 4.0 x 106 100 0 0 0 //9 A.n. 1.4 X 10 6 100 0 0 0 C.a. 6.4 x 106 100 0 0 0 DMDM Hydantoin 0.15% E.c. 3.4 x 106 100 0 0 0 (Room Temperature) S.a. 8.5 x 106 100 0 0 0 P.a. 4.0 x 106 100 0 0 0 (0) denotes no growth Table VIII Henna Cream Conditioner The mold (A.n.) and yeast (C.a.) were both eliminated in the control sample as well as the other samples in the first 28 day preservative test. However, sample//11 (Parabens) failed due to bacteria growth. The DMDM Hydantoin and Quaternium-15 adequately preserved the Henna Cream formulation. DMDM Hydantoin was still active as shown by the second 28 day rechallenge test. Sample//14 was more efficient with the mold (A.n.) and yeast (C.a.), as expected, due to the higher use concentration.

92 JOURNAL OF THE SOCIETY OF COSMETIC CHEMISTS Table VIII Henna Cream Conditioner Preservative System Organism and Initial Count Microorganism Count Per Gram of Product 7 Day 14 Day 21 Day 28 Day Twenty-eight Day Preservative Test ff10 A.n. 1.4 x 106 100 0 0 0 C.a. 6.4 x 106 100 80 0 0 None E.c. 3.4 x 106 4.0 x 105 1.0 x 107 2.8 x 108 3.5 x 108 S.a. 8.5 X 106 5.9 X 105 8.0 X 105 8.5 X 106 4.2 X 107 P.a. 4.0 x 10 6 2.8 x 10 5 9.0 X 107 9.0 X 108 1.0 X 109 fill A.n. 1.4 X 106 100 0 0 0 C.a. 6.4 x 106 100 0 0 0 Methylparaben 0.1% E.c. 3.4 x 106 6.0 x 105 9.0 x 107 5.2 x 108 5.0 x 109 Propylparaben 0.1% S.a. 8.5 x 106 1.9 x 10 5 7.2 x 106 6.4 x 106 8.4 x 108 P.a. 4.0 x 106 1.5 x 106 8.9 X 106 9.0 x 107 2.4 x 108 ff12 A.n. 1.4 X 106 100 0 0 0 C.a. 6.4 x 106 100 8 0 0 Quaternium-15 0.15% E.c. 3.4 x 106 100 0 0 0 S.a. 8.5 X 106 100 0 0 0 P.a. 4.0 x 106 100 0 0 0 ff13 A.n. 1.4 X 106 200 0 0 0 C.a. 6.4 X 10 6 100 90 0 0 DMDM Hydantion 0.25% E.c. 3.4 x 106 100 0 0 0 (Room Temperature) S.a. 8.5 x 106 100 0 0 0 P.a. 4.0 x 106 100 0 0 0 //14 A.n. 1.4 x 106 100 0 0 0 C.a. 6.4 x 106 100 10 0 0 DMDM Hydantoin 0.50% E.c. 3.4 x 106 100 0 0 0 (Room Temperature) S.a. 8.5 x 106 100 0 0 0 P.a. 4.0 x 106 100 0 0 0 Twenty-eight Day Preservative Test (Rechallenge) #13 A.n. 2.1 X 106 350 300 0 C.a. 1.8 X 106 2000 1000 25 DMDM Hydantoin 0.25% E.c. 1.0 x 106 10 x 105 2.0 x 105 1.2 x 103 (Room Temperature) S.a. 1.4 X 10 6 0 0 0 P.a. 4.0 x 106 0 0 0 //14 A.n. 2.1 x 106 500 100 60 C.a. 1.8 x 106 800 175 125 DMDM Hydantoin 0.50% E.c. 1.0 x 106 0 0 0 (Room Temperature) S.a. 1.4 X 106 0 0 0 P.a. 4.0 x 106 0 0 0 0 0 400 0 0 0 0 0 0 0 (0) denotes no growth