92 JOURNAL OF THE SOCIETY OF COSMETIC CHEMISTS Table VIII Henna Cream Conditioner Preservative System Organism and Initial Count Microorganism Count Per Gram of Product 7 Day 14 Day 21 Day 28 Day Twenty-eight Day Preservative Test ff10 A.n. 1.4 x 106 100 0 0 0 C.a. 6.4 x 106 100 80 0 0 None E.c. 3.4 x 106 4.0 x 105 1.0 x 107 2.8 x 108 3.5 x 108 S.a. 8.5 X 106 5.9 X 105 8.0 X 105 8.5 X 106 4.2 X 107 P.a. 4.0 x 10 6 2.8 x 10 5 9.0 X 107 9.0 X 108 1.0 X 109 fill A.n. 1.4 X 106 100 0 0 0 C.a. 6.4 x 106 100 0 0 0 Methylparaben 0.1% E.c. 3.4 x 106 6.0 x 105 9.0 x 107 5.2 x 108 5.0 x 109 Propylparaben 0.1% S.a. 8.5 x 106 1.9 x 10 5 7.2 x 106 6.4 x 106 8.4 x 108 P.a. 4.0 x 106 1.5 x 106 8.9 X 106 9.0 x 107 2.4 x 108 ff12 A.n. 1.4 X 106 100 0 0 0 C.a. 6.4 x 106 100 8 0 0 Quaternium-15 0.15% E.c. 3.4 x 106 100 0 0 0 S.a. 8.5 X 106 100 0 0 0 P.a. 4.0 x 106 100 0 0 0 ff13 A.n. 1.4 X 106 200 0 0 0 C.a. 6.4 X 10 6 100 90 0 0 DMDM Hydantion 0.25% E.c. 3.4 x 106 100 0 0 0 (Room Temperature) S.a. 8.5 x 106 100 0 0 0 P.a. 4.0 x 106 100 0 0 0 //14 A.n. 1.4 x 106 100 0 0 0 C.a. 6.4 x 106 100 10 0 0 DMDM Hydantoin 0.50% E.c. 3.4 x 106 100 0 0 0 (Room Temperature) S.a. 8.5 x 106 100 0 0 0 P.a. 4.0 x 106 100 0 0 0 Twenty-eight Day Preservative Test (Rechallenge) #13 A.n. 2.1 X 106 350 300 0 C.a. 1.8 X 106 2000 1000 25 DMDM Hydantoin 0.25% E.c. 1.0 x 106 10 x 105 2.0 x 105 1.2 x 103 (Room Temperature) S.a. 1.4 X 10 6 0 0 0 P.a. 4.0 x 106 0 0 0 //14 A.n. 2.1 x 106 500 100 60 C.a. 1.8 x 106 800 175 125 DMDM Hydantoin 0.50% E.c. 1.0 x 106 0 0 0 (Room Temperature) S.a. 1.4 X 106 0 0 0 P.a. 4.0 x 106 0 0 0 0 0 400 0 0 0 0 0 0 0 (0) denotes no growth

DMDM HYDANTOIN AS A COSMETIC PRESERVATIVE 93 Table IX Hand Cream All of the preservatives tested separately failed to protect the hand cream from all microorganisms. The Parabens (Sample//16) failed on yeast and bacteria growth. The Quaternium-15 (Sample//17) failed on yeast growth. The DMDM Hydantoin (Sample //18) failed on all microorganisms. However at higher use concentration (Sample//19), the DMDM Hydantoin did control all the bacteria microorganisms. Table IX Hand Cream Microorganism Count Per Gram of Product Organism and Initial Preservative System Count 7 Day 14 Day 21 Day 28 Day Twenty-eight Day Preservative Test //15 A.n. 1.4 x 10 6 2.2 x 10 4 3.0 x 10 4 2.8 X 10 4 2.6 x 10 4 C.a. 6.4 X 106 2.3 ( 106 1.4 X 107 1.0 x 107 1.8 X 107 None E.c. 3.4 x 106 1.2 x 106 1.4 x 108 2.0 x 108 2.8 x 108 S.a. 8.5 x 106 2.4 x 106 8.0x 10 ? 2.6 x 108 3.0 x 109 P.a. 4.0 x 106 3.0 x 106 5.5 x 107 6.0 x 107 7.0 x 108 //16 A.n. 1.4 x 106 100 100 0 0 Methylparaben C.a. 6.4 x 106 3.5 ( 106 7.7 ( 106 8.0 x 107 2.5 x 108 0.1% E.c. 3.4 x 106 5.0 x 106 1.7 x 107 2.4 x 107 4.1 x 108 Propylparaben S.a. 8.5 x 106 1.8 x 106 2.3 x 107 1.5 x 108 2.4 x 108 0.1% P.a. 4.0 x 10 6 9.0 x 10 6 1.2 x 10 7 3.1 x 10 8 4.5 x 10 8 //17 A.n. 1.4 x 106 100 0 0 0 C.a. 6.4 x 106 1.7 x 106 3.9 x 105 1.7 x 107 3.0 x 107 Quaternium-15 E.c. 3.4 x 106 100 0 0 0 0.15% S.a. 8.5 x 106 100 0 0 0 P.a. 4.0 x 106 100 0 0 0 //18 A.n. 1.4 x 106 Overgrown Overgrown Overgrown Overgrown Mold Mold Mold Mold C.a. 6.4 x 106 5.0 x 106 5.5 x 105 6.0 x 106 4.2 x 107 DMDM Hydantoin E.c. 3.4 x 106 8.5 x 106 9.0 x 105 2.0 x 106 2.6 x 106 0.2% S.a. 8.5 x 106 2.4 x 106 4.0 x 103 3.0 x 103 200 P.a. 4.0 x 106 7.0 x 106 2.0 x 106 4.0 x 106 5.0 x 106 //19 A.n. 1.4 x 106 Overgrown Overgrown Overgrown Overgrown Mold Mold Mold Mold C.a. 6.4 X 106 6.1 X 105 5.8 X 106 2.4 x 105 2.9 X 105 DMDM Hydantoin E.c. 3.4 x 106 100 0 0 0 0.4% S.a. 8.5 x 106 100 0 0 0 P.a. 4.0 x 106 100 0 0 0 (0) denotes no growth