62 JOURNAL OF THE SOCIETY OF COSMETIC CHEMISTS REFERENCES (19) (20) (21) (22) (23) (24) (25) (26) (1) K. J. McGinley, R. R. Marpies, and G. Piewig, A method for visualizing and quantitating the desquamation portion of the human stratum corneum, J. Invest. Dermatol., 53, 107-111 (1969). (2) E. H/51zle and G. Plewig, Effect of dermatitis, stripping and steroids on the morphology of corneo- cytes. A new bioassay, J. Invest. Dermatol., 68, 350-356 (1977). (3) S. Lee, Y. K. Park, Y. K. Kim, and J. S. Kang, An experimental study on corneocytes of acutely and chronically irritated skin, Arch. Dermatol. Res., 275, 49-52 (1983). (4) H. Goldschmidt, Surface area measurements of psoriatic corneocytes: Effects of intralesional steroid therapy, J. Invest. Dermatol., 73, 558-560 (1979). (5) M. T. Hojyo-Tomoka and A.M. Kligman, Does cellophane stripping remove the horny layer? Letters to the editor, Arch. Dermatol., 106, 767-768 (1972). (6) R. Hunter, H. Pinkus, and C. H. Steele, Examination of the epidermis by the strip method. III. The number of keratin cells in the human epidermis, J. Invest. Dermatol., 27, 31-34 (1956). (7) E. H/51zle, J. Park, and G. Plewig, EinfluB verschiedener Glukokortikoide und ihrer Grundlagen auf die Korneozyten der normalen Epidermis, Akt. Dermatol., 6, 75-81 (1980). (8) M. Papini, P. Mariani, and P. Calandra, Topical motretinid treatment of skin diseases with patho- logical keratinization, Ann. It. Derm. Clin. Sper., 37, 175-180 (1983). (9) E H/51zle, G. Plewig, and A. Ledolter, "Corneocyte Exfoliative Cytology: A Model to Study Normal and Diseased Stratum Corneum," in Skin Models, 1st ed., R. Marks, and G. Plewig, Eds. (Springer Verlag, Berlin, Heidelberg, 1986), pp. 183-193. (10) S. Herrmann, E. Scheuber, and G. Plewig, "Exfoliative Cytology: Effects of the Seasons," in Stratum Corneum, 1st ed., R. Marks and G. Plewig, Eds. (Springer Verlag, Berlin, Heidelberg, 1983), pp. 181-185. (11) H. Goldschmidt and A.M. Kligman, Exfoliative cytology of human horny layer, Arch. Dermatol., 96, 572-576 (1967). (12) W. Breiner, E. $cheuber, and G. Piewig, "Effects of Isotretinoin (13-cis Retinoic Acid, Ro 4-3780) Treatment on Exfoliative Cytology," in Stratum Corneum, 1st ed., R. Marks and G. Piewig, Eds. (Springer Verlag, Berlin, Heidelberg, 1983), pp. 222-226. (13) G. Piewig, Regional differences of cell sizes in the human stratum corneum. Part II: Effects of sex and age, J. Invest. Dermatol., 54, 19-23 (1970). (14) G. Piewig and R. R. Marpies, Regional differences of cell size in the human stratum corneum, J. Invest Dermatol., 54, 13-18 (1970). (15) R. Marks, Epidermal and corneocyte size changes with age, Brit. Jo Dem., 102, 738-739 (1980). (16) H. Germann, W. Barran, and G. Piewig, Morphology of corneocytes from human nail plates, Jo Invest. Dermatol., 74, 115-118 (1980). (17) K. A. Holbrook and G. F. Odland, Regional differences in the thickness (cell layers) of the human stratum corneum: An ultrastructural analysis, J. Invest. Dermatol., 62, 415-422 (1974). (18) G. Piewig, E. Scheuber, B. Reuter, and W. Waidelich, "Thickness of Corneocytes," in Stratum Corneum, 1st ed., R. Marks and G. Piewig, Eds., (Springer Verlag, Berlin, Heidelberg, 1983), pp. 171-173. F. Allegra and C. De Panfilis, An in vivo method of studying the kinetics of cell proliferation in normal human epidermis, Acta Dermatovener (Stockholm), 54, 87-90 (1974). H. Baker and A.M. Kligman, Technique for estimating turnover time of human stratum corneum, Arch. Dermatol., 95, 408-411 (1967). P. Corcuff, G. Delesalle, and H. Schaefer, Quantitative aspect of corneocytes,J. Soc. Cosmet. Chem., 33, 1-7 (1982). W. L. Epstein and H. I. Maibach, Cell renewal in human epidermis, Arch. Dermatol., 92, 462-468 (1965). H. Goldschmidt and A.M. Kligman, Desquamation of the human horny layer, Arch. Dermatol., 95, 583- 586 (1967). H. Pinkus, Examination of the epidermis by the strip method of removing horny layers. Observation of thickness of the horny layer and on mitotic activity after stripping. J. Invest. Dermatol., 16, 383-386 (1951). H. Pinkus, Examination of the epidermis by the strip method: Biometric data on regeneration of the human epidermis, J. Invest Dermatol., 19, 431-447 (1952). G. Piewig and A. M. Kligman, Zellkinetische Untersuchungen bei Kopfschuppenerkrankung (Pity- riasis simplex capillitii), Arch. Klin. Exp. Derre., 236, 406-421 (1970).

CYTOLOGY OF STRATUM CORNEUM 63 (27) (28) (29) (30) (31) (32) (33) (34) (35) (36) (37) (38) (39) (40) (41) (42) (43) (44) (45) (46) (47) (48) (49) (5O) (51) (52) G. Piewig, Kopfschuppen und ihre Behandlung, Kosmetologie, 3, 109-114 (1971). S. Shuster, The aetiology of dandruff and the mode of action of therapeutic agents, Brit. J. Derm., 111, 235-242 (1984). G. L. Grove and A. M. Kligman, "Corneocytes' Size as an Indirect Measure of Epidermal Prolifera- tive Activity," in Stratum Corneum, 1st ed., R. Marks and G. Piewig, Eds. (Springer Verlag, Berlin, Heidelberg, 1983), pp. 191-195. R. Marks and S. P. Barton, "The Significance of the Size and Shape of Corneocytes," in Stratum Corneum, 1st ed., R. Marks and G. Plewig, Eds. (Springer Verlag, Berlin, Heidelberg, 1983), pp. 161 - 170. A.M. Kligman, "The Biology of the Stratum Corneum," in The Epidermis, W, Montagna and W. C. Lobitz, Eds. (Academic Press, New York, London, 1964), pp. 387-433. R. Marks, Measurement of biological ageing in human epidermis, Brit. J. Derm., 104, 627-633 (1981). G. Piewig, O. Braun-Falco, W. K16vehorn, and Ch. Luderschmidt, Isotretinoin zur 6rtlichen Be- handlung von Akne und Rosazea sowie tierexperimentelle Untersuchungen mit Isotretinoin und Arotinoid, Hautarzt, 37, 138-141 (1986). M. Sakamoto and S. M. Puhvel, Effect of topical application of 13-cis-retinoic acid on skin of hairless rats,J. Invest. Dermatol., 80, 356 (Abstr.) (1983). P. Agache, J. P. Boyer, and R. Laurent, Biomechanical properties and microscopic morphology of human stratum corneum incubated on a wet pad in vitro, Arch. Derre. Forsch., 246, 271-283 (1973). R. L. Anderson, J. M. Cassidy, J. R. Hansen, and W. Yellin, The effect of in vivo occlusion on human stratum corneum hydration-dehydration in vitro, J. Invest. Dermatol., 61, 375-379 (1973). R. L. Anderson and J. M. Cassidy, Variations in physical dimensions and chemical composition of human stratum corneum, J. Invest. Dermatol., 61, 30-32 (1973). H. Baker and C. P. Blair, Cell replacement in the human stratum corneum in old age, Brit. J. Derm., 80, 367-372 (1968). H. Baker and A.M. Kligman, A simple in vivo method for studying the permeability of the human stratum corneum, J. Invest. Dermatol., 48, 273-274 (1967). H. Baker and A.M. Kligman, Measurement of transepidermal water loss electrical hygrometry, Arch. Dermatol., 96, 441-452 (1967). M. J. Bartek, J. A. LaBudde, and H. I. Maibach, Skin permeability in vivo: Comparison in rat, rabbit, pig and man, J. Invest. Dermatol., 58, 114-123 (1972). S. P. Barton, C. S. King, R. Marks, and S. Nicholls, A technique for studying the structural detail of isolated human corneocytes, Brit. J. Derm., 102, 63-73 (1980). F. Barry and V. Duzee, The influence of water content, chemical treatment and temperature on the theological properties of stratum corneum, J. Invest. Dermatol., 71, 140-144 (1978). C. Blair, Morphology and thickness of the human stratum corneum, Brit. J. Derm., 80, 430-436 (1968). C. Blair, The thickness of the stratum corneum in acne vulgaris, Brit. J. Derre., 80, 516- 519 (1968). C. Blair, The action of a urea-lactid acid ointment in ichthyosis. With particular reference to the thickness of the horny layer, Brit. J. Derm., 94, 145-153 (1976). I. H. Blank and R. K. Dawes, The water content of the stratum corneum. IV. The importance of water in promoting bacterial multiplication on cornified epithelium, J. Invest. Dermatol., 31, 141-145 (1958). W. Bollag, Therapy of epithelial tumors with an aromatic retinoic acid analog, Chemotherapy, 21, 236-247 (1975). W. S. Bullough and B. E. Laurence, The production of epidermal cells, Symp. Zool. Soc. Lond., 12, 1-23 (1964). O. Braun-Falco and M. Rupec, Die Verteilung der sauren Phosphatase bei normaler und psori- atischer Verhornung. Eine elektronenmikros-kopisch-cytochemische Untersuchung, Dermatologica, 134(4) 225-241 (1967). O. Braun-Falco, E. Christophers, and A. Kurban, Abnormes Verhalten der epidermalen Regenera- tion bei Patienten mit Psoriasis vulgaris, Arch. Klin. Exp. Derm., 229, 276-284 (1967). W. S. Bullough, The control of epidermal thickness, Parts I and II, Brit. J. Derm., 87, 187-354 (1972).