310 JOURNAL OF COSMETIC SCIENCE It is apparent from this data that avocado sterols can effect the production of a known potent proinflammatory mediator of cellular migration, IL-8 (1,2,3). This data suggests that avocado sterols, like other steroids, have a profound effect on chronic inflammatory mediators. The use of phytosterols, specifically avocado derived, in skincare products could combat the deleterious effects seen in chronic inflammatory dermatological conditions such as atopic dermatitis and psoriasis by down regulating IL-8 production from damaged keratinocytes. Acknowledgments The authors wish to thank Jerry Burke, Peter, Cade and Robert Comber, Ph.D. for their numerous helpful discussions throughout this project. All formulas used were made by Robert Lanese in Technical Services at Croda Inc. Kathy Koukouras was the recipient of a Croda Summer 2000 Internship. References 1) The Cytokine Handbook, Thomson, AW, 3 rd Edition, Academic Press, London, UK. 1017 pp. Illustrated, (1998). 2) Konstantinova, NV, Duong, D-MT, Remenyik, E, Hazarika, P, Chuang, A, and Duvic, M. Interleukin-8 is Induced in Skin Equivalents and is Highest in Those Derived from Psoriatic Fibroblasts. J Invest Dermatol, 107(4): 615-621, (1996). 3) Wen, YJ, Chattedee, AD, Raychaudhuri, SP, Raychaudhuri, SK and Farber, EM. Mast Cell Density and IL-8 Expression in Nonlesional and Lesional Psoriatic Skin. Int. d. Derm. 40, 699-703, (2001). 4) MatTek Corporation, EpiDerm (EPI-200) Product Guide MTT Assay, (2000). 5) Endogen Human ELISA IL-lct Kit, (2000). 6) Endogen Human ELISA IL-8 Kit, (2000). 7) Cayman Chemicals Bicyclo Prostaglandin E2 EIA Kit Protocol, (2000). Figure 1: Avocado SteroIs Protect Cells from UVR Damage • Lotion plus Avocado •-LotionsNone - a -UV•None ]

2002 ANNUAL SCIENTIFIC SEMINAR 311 Figure 2: Avocado Sterois Don't Decrease the Production of PGE2 Due to UVB Exposure t2 24 Time (Hours) ß Avocado Lo•on • L•(ion m UV13 A•Ot• E•No Treatment ] Figure 3: Avocado Sterois Decrease the Production of IL-lcr Due to UVB Exposure tooo lOOO 14oo ioo 4o0 o 24 [ i• AvoCado LO•(xt (• Lotion 3 UVB • NO Trlamlent ] Figure 4: Avocado SteroIs Decrease the Production of iL-8 Due to UVB Exposure Time (Hours)