Figure
5.
The
effects
of
HCP
on
Wnt/β-catenin
pathway.
Quantification
of
β-Catenin,
p-GSK-3βand
MITF
protein
levels
in
PIG1
(A)
and
MelanA
(B)
cells
by
Image
J
software
for
Figure
4A
and
4B.
Graph
of
β
positive
nuclei
percentage
quantification
in
PIG1
(C)
and
MelanA
(D)
cells
for
Figure
4C
and
4D.
***p
0.001,
ET-1
versus
dimethyl
sulfoxide
(DMSO)
control
#
p
0.05,
##
p
0.01,
###
p
0.01,
HCP
versus
ET-1.
168 JOURNAL OF COSMETIC SCIENCE

Figure
6.
The
effects
of
HCP
on
TYR
expression
and
activity.
(A)
MelanA
cells
were
pretreated
with
20
nM
ET-1
for
24h,
then
treated
with
different
concentrations
of
HCP
for
24h.
TYR
expression
were
determined
by
Western
blot
analysis.
(B)
Quantification
of
tyrosianse
protein
expression
in
MelanA
cells
by
Image
J
software.
(C)
The
activity
of
tyrosianse
in
MelanA
cells.
Data
were
expressed
mean
±
SD
from
3
independent
experiments
(n
=
3).
**p
0.01
ET-1
vs
control
#
p
0.05,
##
p
0.01,
###
p
0.01,
HCP
versus
ET-1.
169 Hydrolyzed Conchiolin Protein Inhibits Melanin