185 POLYPODIUM LEUCOTOMOS EXTRACT
fluorescent microscope and analyzed with ZEN 2012 program (Zeiss). DNA fragmentation
was quantitated according to comet tail length.
STATISTICS
Data are mean ± SD from at least two culture independent dishes conditions representative
of two or three independent experiments, unless stated otherwise. P values were calculated
with two-tailed Student’s t-test (Excel). P values of 0.05 were considered significant. Most
analyses were repeated in two different epidermal strains from two different individuals
with similar results.
ACKNOWLEDGMENTS
We thank Alicia Noriega, Laura Ceballos, Marta Cagigas, Ángel Saiz, and Lorena García-
Gaipo for technical assistance. The work was in part funded by Cantabria Labs and in
part by grant ISCIII-FIS/FEDER PI20/00880 (A.G.). Lucía San Juan was supported by a
fellowship from Universidad de Cantabria/Idival, PREVAL 19/06.
CONFLICT OF INTEREST
S.G. is a consultant for Cantabria Labs, and A.Gu., A.D.R., and A.R.L. belonged to the
Innovation and Development Department at Cantabria Labs (at the time of the work), which
produced Fernblock® technology. The remaining authors declare no conflict of interest.
CONTRIBUTIONS
A.G.: data curation, experimental design, analyses, funding, figure making, manuscript
writing, and validation A.Gu. and A.D.R.: experimental design, analyses, funding,
manuscript validation S.G.: analyses, manuscript validation A.R.L.: analyses, manuscript
writing, and validation L.S.J.: data curation, figure making, manuscript writing, and
validation.
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