146 JOURNAL OF COSMETIC SCIENCE (a) 100 c::J In PBS � loaded In DOPE:CHEMS 80 0 100 100 50 Concentration ofNB-DNJ (µM) (b) 100 c::J In PBS loaded In DOPE:CHEMS 80 -��-------....-.--- fl) ·- fl) ,....., tiu- 60 fl) u ·= '+- 1 40 ------.,�-- f� 20 0 100 100 50 Concentration of DNJ (µM) Figure 3. Melanin synthesis reduced by NB-DNJ loaded (a) and DNJ loaded (b) pH-sensitive liposomes in comparison with each of free N-glycosylation inhibitors.

(a) (b) � = Q u � = Q u DELIVERY ACTIVITY OF pH-SENSITIVE LIPOSOMES 147 PC:CHEMS liposome 10• FU-Height w• FU-Height Figure 4. Histograms of cellular fluorescent intensity of HM3KO cells treated with PC:CHEMS and DOPE:CHEMS liposomes containing 1 % fluorescent-DHPE (a) and treated with calcein-loaded DOPE: CHEMS and PC:CHEMS liposomes (b) as measured by FACS. ery and the internalization mediated by pH-sensitive DOPE:CHEMS liposomes. DOPE: fluorescein-DHPE:PEG-5 rapeseed sterol (L5) liposome was used as a control for pH- insensitive liposomes. Cellular uptake behavior of the pH-sensitive liposomes was moni- tored by using 1 % fluorescein-DHPE incorporated in the liposomes. Simultaneously,

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