195 IN VIVO ELONGATION OF TOPICALLY APPLIED FATTY ACIDS
formulations was incorporated into ceramides, as measured by the release of sphingosine
via hydrolysis of ceramides The concept of d31-PA integration into ceramides via the de novo
pathway for ceramide and sphingosine synthesis was introduced by the authors at the AAD
conference.25
0
C18:0 C20:0
Deutered fatty acids detected in skin
Deutered fatty acids detected in skin
d31PA concentration
C22:0 C24:0
10
20
30
40
50
60
70
80
90
100
A
B
10
20
30
40
50
60
70
80
90
100
2% 0.75% 0.25% 0.05%
C18:0 C20:0 C22:0 C24:0
0
Figure 3. Percentage of participants demonstrating conversion of different levels of d31-PA (C16:0) into
elongated FFAs following application of a body wash formulation twice daily for four weeks on the arm (A)
and leg (B). Detectable levels of elongated FFAs (C20:0 to C24:0) were found in tape strip samples for all body
wash d31-PA concentrations.
d31-PA FFA, free fatty acid.
Participants
demonstrating
conversion
of
the
d31PA
concentration
applied
(%)
Participants
demonstrating
conversion
of
the
d31PAconcentration
applied
(%)
formulations was incorporated into ceramides, as measured by the release of sphingosine
via hydrolysis of ceramides The concept of d31-PA integration into ceramides via the de novo
pathway for ceramide and sphingosine synthesis was introduced by the authors at the AAD
conference.25
0
C18:0 C20:0
Deutered fatty acids detected in skin
Deutered fatty acids detected in skin
d31PA concentration
C22:0 C24:0
10
20
30
40
50
60
70
80
90
100
A
B
10
20
30
40
50
60
70
80
90
100
2% 0.75% 0.25% 0.05%
C18:0 C20:0 C22:0 C24:0
0
Figure 3. Percentage of participants demonstrating conversion of different levels of d31-PA (C16:0) into
elongated FFAs following application of a body wash formulation twice daily for four weeks on the arm (A)
and leg (B). Detectable levels of elongated FFAs (C20:0 to C24:0) were found in tape strip samples for all body
wash d31-PA concentrations.
d31-PA FFA, free fatty acid.
Participants
demonstrating
conversion
of
the
d31PA
concentration
applied
(%)
Participants
demonstrating
conversion
of
the
d31PAconcentration
applied
(%)
