Figure
5.
The
calibration
curves
of
Cer-NP
in
the
range
of
80
µg/mL
and
480
µ
(=
3).
285 CERAMIDE-NP IN SKIN-SIMULATING LIPOSOME FORMULATIONS

286 JOURNAL OF COSMETIC SCIENCE
CONCLUSION
Ceramides are used in the composition of cosmetic or dermatological products to restore the
skin barrier. However, the quantification methods of ceramides in liposome formulations
including phosphatidylcholines and cholesterol have been relatively time-consuming
because the hydrophobicity of ceramides, depending on fatty acyl chain length, can cause
turbidity in various alcoholic solvents. In addition, the similarity of the physicochemical
structure of ceramides and lipid excipients used in the composition of liposome formulations
complicates their separation and quantification in the analysis. Therefore, a simple analytical
method to selectively determine Cer-NP in liposome formulations is still required. In
this work, a simple, cost-effective, and selective chromatographic method was developed
to quantify simultaneously Cer-NP in the skin-stimulating liposomes, which has been
previously formulated as a promising carrier for recovering the skin barrier previously.
Then, the method was validated in terms of its specificity, linearity, accuracy, precision,
LOD, and LOQ. The results showed that the HPLC method developed could be useful to
analyze simultaneously Cer-NP in the liposome formulations.
CONFLICT OF INTEREST
The authors declare that there is no conflict of interest regarding the publication of this paper.
ACKNOWLEDGMENTS
This research was supported by Istanbul University Scientific Research Project (Project
number: 37152). The authors would also like to thank “Evonik Industries” and “Lipoid
GmBH” for gently providing Ceramide NP and Phospholipon 90G, respectively.
REFERENCES
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Accessed May 1, 2022. https://pubmed.ncbi.nlm.nih.gov/28210396/
(3) Vovesná A, Zhigunov A, Balouch M, Zbytovská J. Ceramide liposomes for skin barrier recovery:
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Table IV
The Results of Accuracy, Intra-, and Inter-Day Analysis
Intraday (CV, %)Interday (CV, %)Accuracy (%)
Analyte Level 1 Level 2 Level 3 Level 1 Level 2 Level 3 Level 1 Level 2 Level 3
Cer-NP 1.03 0.99 1.66 3.01 1.94 4.41 110.21±1.13 107.83±1.07 97.03±1.61