287 CERAMIDE-NP IN SKIN-SIMULATING LIPOSOME FORMULATIONS
(5) Khiste SK, Hosain SB, Dong Y, et al. Incorporation of fluorescence ceramide-based HPLC assay for
rapidly and efficiently assessing glucosylceramide synthase in vivo. Sci Rep 2017 7. 2017 7(1):1–13.
doi:10.1038/s41598-017-03320-9
(6) Tessema EN, Gebre-Mariam T, Frolov A, Wohlrab J, Neubert RHH. Development and validation of LC/
APCI-MS method for the quantification of oat ceramides in skin permeation studies. Anal Bioanal Chem.
2018 410(20):4775–4785. doi:10.1007/S00216-018-1162-Z
(7) Fujiwara A, Morifuji M, Kitade M, et al. Age-related and seasonal changes in covalently bound ceramide
content in forearm stratum corneum of Japanese subjects: determination of molecular species of
ceramides. Arch Dermatol Res. 2018 310(9):729–735. doi:10.1007/S00403-018-1859-Z
(8) Gaudin K, Chaminade P, Baillet A, et al. Contribution to liquid chromatographic analysis OF cutaneous
ceramides. Journal of Liquid Chromatography &Related Technologies. 1999 22(3):379–400. doi:10.1081/
JLC-100101667
(9) Iwamori M, Costello C, Moser HW. Analysis and quantitation of free ceramide containing nonhydroxy
and 2-hydroxy fatty acids, and phytosphingosine by high-performance liquid chromatography. J
Lipid Res. 1979 20(1):86–96. Accessed October 12, 2020. https://europepmc.org/article/med/438657.
doi:10.1016/S0022-2275(20)40654-6
(10) Gildenast T, Lasch J. Isolation of ceramide fractions from human stratum corneum lipid extracts by
high-performance liquid chromatography. Biochim Biophys Acta. 1997 1346(1):69–74. doi:10.1016/
S0005-2760(97)00019-2
(11) Zhou Q, Zhang L, Fu XQ, Chen GQ. Quantitation of yeast ceramides using high-performance liquid
chromatography-evaporative light-scattering detection. J Chromatogr B Analyt Technol Biomed Life Sci.
2002 780(1):161–169. doi:10.1016/S1570-0232(02)00466-X
(12) Meyer VR. Practical High-performance Liquid Chromatography. 5th ed. Practical High-Performance
Liquid Chromatography. Published online April 9, 2010:1-402. doi:10.1002/9780470688427.
(13) Şahin Bektay H, Kahraman E, Güngör S. Design of skin-simulating nanoformulations for ceramide
replacement in the skin: a preliminary study. Maced Pharm Bull. 2020 66(1):101–102. doi:10.33320/
maced.pharm.bull.2020.66.03.050
(14) Kahraman E, Güngör S, Özsoy Y. Potential enhancement and targeting strategies of polymeric and lipid-
based nanocarriers in dermal drug delivery. Ther Deliv. 2017 8(11):967–985. doi:10.4155/TDE-2017-0075
(15) Tessema EN, Gebre-Mariam T, Paulos G, Wohlrab J, Neubert RHH. Delivery of oat-derived
phytoceramides into the stratum corneum of the skin using nanocarriers: formulation, characterization
and in vitro and ex-vivo penetration studies. Eur J Pharm Biopharm. 2018 127:260–269. doi:10.1016/j.
ejpb.2018.02.037
(16) ICH, Topic Q. 2 (R1) validation of analytical procedures: text and methodology Step 5 NOTE FOR
GUIDANCE ON VALIDATION OF ANALYTICAL PROCEDURES: TEXT AND METHODOLOGY
(CPMP/ICH/381/95) APPROVAL BY CPMP. Date For Coming Oper. Published online. November
1994 1995. Accessed May 1, 2022. https://www.ema.europa.eu/en/documents/scientific-guideline/
ich-q-2-r1-validation-analytical-procedures-text-methodology-step-5_en.pdf
(17) Q2B validation of analytical procedures: methodology. Accessed September 17, 2023. https://www.
fda.gov/regulatory-information/search-fda-guidance-documents/q2b-validation-analytical-procedures-
methodology. FDA.
(18) Angelo T, Pires FQ, Gelfuso GM, da Silva JKR, Gratieri T, Cunha-Filho MSS. Development and
validation of a selective HPLC-UV method for thymol determination in skin permeation experiments. J
Chromatogr B Analyt Technol Biomed Life Sci. 2016 1022:81–86. doi:10.1016/j.jchromb.2016.04.011
(19) Slotte JP, Yasuda T, Engberg O, et al. Bilayer interactions among unsaturated phospholipids, sterols, and
ceramide. Biophys J. 2017 112(8):1673–1681. doi:10.1016/J.BPJ.2017.03.016
(20) Castro BM, Prieto M, Silva LC. Ceramide: a simple sphingolipid with unique biophysical properties. Prog
Lipid Res. 2014 54(1):53–67. doi:10.1016/J.PLIPRES.2014.01.004
(21) Neubert RHH, Sonnenberger S, Dobner B, et al. Controlled penetration of a novel dimeric ceramide into
and across the stratum corneum using microemulsions and various types of semisolid formulations. Skin
Pharmacol Physiol. 2016 29(3):130–134. doi:10.1159/000445776
(5) Khiste SK, Hosain SB, Dong Y, et al. Incorporation of fluorescence ceramide-based HPLC assay for
rapidly and efficiently assessing glucosylceramide synthase in vivo. Sci Rep 2017 7. 2017 7(1):1–13.
doi:10.1038/s41598-017-03320-9
(6) Tessema EN, Gebre-Mariam T, Frolov A, Wohlrab J, Neubert RHH. Development and validation of LC/
APCI-MS method for the quantification of oat ceramides in skin permeation studies. Anal Bioanal Chem.
2018 410(20):4775–4785. doi:10.1007/S00216-018-1162-Z
(7) Fujiwara A, Morifuji M, Kitade M, et al. Age-related and seasonal changes in covalently bound ceramide
content in forearm stratum corneum of Japanese subjects: determination of molecular species of
ceramides. Arch Dermatol Res. 2018 310(9):729–735. doi:10.1007/S00403-018-1859-Z
(8) Gaudin K, Chaminade P, Baillet A, et al. Contribution to liquid chromatographic analysis OF cutaneous
ceramides. Journal of Liquid Chromatography &Related Technologies. 1999 22(3):379–400. doi:10.1081/
JLC-100101667
(9) Iwamori M, Costello C, Moser HW. Analysis and quantitation of free ceramide containing nonhydroxy
and 2-hydroxy fatty acids, and phytosphingosine by high-performance liquid chromatography. J
Lipid Res. 1979 20(1):86–96. Accessed October 12, 2020. https://europepmc.org/article/med/438657.
doi:10.1016/S0022-2275(20)40654-6
(10) Gildenast T, Lasch J. Isolation of ceramide fractions from human stratum corneum lipid extracts by
high-performance liquid chromatography. Biochim Biophys Acta. 1997 1346(1):69–74. doi:10.1016/
S0005-2760(97)00019-2
(11) Zhou Q, Zhang L, Fu XQ, Chen GQ. Quantitation of yeast ceramides using high-performance liquid
chromatography-evaporative light-scattering detection. J Chromatogr B Analyt Technol Biomed Life Sci.
2002 780(1):161–169. doi:10.1016/S1570-0232(02)00466-X
(12) Meyer VR. Practical High-performance Liquid Chromatography. 5th ed. Practical High-Performance
Liquid Chromatography. Published online April 9, 2010:1-402. doi:10.1002/9780470688427.
(13) Şahin Bektay H, Kahraman E, Güngör S. Design of skin-simulating nanoformulations for ceramide
replacement in the skin: a preliminary study. Maced Pharm Bull. 2020 66(1):101–102. doi:10.33320/
maced.pharm.bull.2020.66.03.050
(14) Kahraman E, Güngör S, Özsoy Y. Potential enhancement and targeting strategies of polymeric and lipid-
based nanocarriers in dermal drug delivery. Ther Deliv. 2017 8(11):967–985. doi:10.4155/TDE-2017-0075
(15) Tessema EN, Gebre-Mariam T, Paulos G, Wohlrab J, Neubert RHH. Delivery of oat-derived
phytoceramides into the stratum corneum of the skin using nanocarriers: formulation, characterization
and in vitro and ex-vivo penetration studies. Eur J Pharm Biopharm. 2018 127:260–269. doi:10.1016/j.
ejpb.2018.02.037
(16) ICH, Topic Q. 2 (R1) validation of analytical procedures: text and methodology Step 5 NOTE FOR
GUIDANCE ON VALIDATION OF ANALYTICAL PROCEDURES: TEXT AND METHODOLOGY
(CPMP/ICH/381/95) APPROVAL BY CPMP. Date For Coming Oper. Published online. November
1994 1995. Accessed May 1, 2022. https://www.ema.europa.eu/en/documents/scientific-guideline/
ich-q-2-r1-validation-analytical-procedures-text-methodology-step-5_en.pdf
(17) Q2B validation of analytical procedures: methodology. Accessed September 17, 2023. https://www.
fda.gov/regulatory-information/search-fda-guidance-documents/q2b-validation-analytical-procedures-
methodology. FDA.
(18) Angelo T, Pires FQ, Gelfuso GM, da Silva JKR, Gratieri T, Cunha-Filho MSS. Development and
validation of a selective HPLC-UV method for thymol determination in skin permeation experiments. J
Chromatogr B Analyt Technol Biomed Life Sci. 2016 1022:81–86. doi:10.1016/j.jchromb.2016.04.011
(19) Slotte JP, Yasuda T, Engberg O, et al. Bilayer interactions among unsaturated phospholipids, sterols, and
ceramide. Biophys J. 2017 112(8):1673–1681. doi:10.1016/J.BPJ.2017.03.016
(20) Castro BM, Prieto M, Silva LC. Ceramide: a simple sphingolipid with unique biophysical properties. Prog
Lipid Res. 2014 54(1):53–67. doi:10.1016/J.PLIPRES.2014.01.004
(21) Neubert RHH, Sonnenberger S, Dobner B, et al. Controlled penetration of a novel dimeric ceramide into
and across the stratum corneum using microemulsions and various types of semisolid formulations. Skin
Pharmacol Physiol. 2016 29(3):130–134. doi:10.1159/000445776
