INTERACTIONS OF COSMETIC PIGMENTS WITH PRESERVATIVES 87 PREPARATION OF Na AND K SALTS OF P-HYDROXYBENZOIC ACID ESTERS A column (1.5 cm X 30 cm) was packed with a slurry of ion-exchange resin (Amberlyst XN-1004) in CH3OH and washed with a large quantity of methanolic NaC1 or KC1. p-Hydroxybenzoic acid ester was placed on the column and eluted with methanol. Elutate was concentrated by evaporation. Whether or not the replacement of phenolic hydrogen with Na or K was complete was determined by •H-NMR spectroscopy. RESULTS AND DISCUSSION DETERMINATION OF THE BACTERICIDAL ACTIVITIES OF THE PRESERVATIVES IN THE PRESENCE OF COSMETIC PIGMENTS Figure 3 shows IDs for various pigments using S. aureus. The bactericidal activity of MP was significantly inhibited in the presence of all pigments except kaolinite and silica alumina, whereas the bactericidal activity of BP was not significantly inactivated by any of the pigments tested. The degree of inactivating activity against MP, EP, and PP was highest for ultramarine blue followed by talc, titanium dioxide (R), titanium dioxide (A), red oxide of iron, and yellow oxide of iron, in decreasing order. Red oxide of iron Yellow oxide of iron Titanium dioxide (A) Titanium dioxide (R) Talc Ultramarine blue Silica alumina Kaolinite • against MP v///, against EP -• against PP ß .• against BP i i 10 -s 10 -a 10 -• I.D. Figure 3. Effects of pigments on the bactericidal activity of p-hydroxybenzoic acid esters against Staphylo- coccus aureus.

88 JOURNAL OF THE SOCIETY OF COSMETIC CHEMISTS It is interesting that ultramarine blue and talc were by far the strongest inactivators of MP, EP, and PP, and yet they had little effect on the activity of BP. Three other lots of ultramarine blue were examined for their IDs using S. aureus. As shown in Figure 4, no differences were observed among the three lots. So, it was con- cluded that the inactivation of MP, EP, and PP by ultramarine blue was brought about by its characteristic properties. Figure 5 shows the IDs of ultramarine blue using the other three microorganisms, that is, E. coli, P. aeruginosa, and C. albicans. Ultramarine blue was also found to exhibit the strongest inactivation to the bactericidal action of MP, the same as with S. aureus. The bactericidal actions of EP and PP were inhibited to some extent by ultramarine blue, EP more so than PP. From these results, it is postu- lated that inactivation of p-hydroxybenzoic acid esters (parabens) by ultramarine blue is a phenomenon of general occurrence. DETERMINATION OF ADSORBED PRESERVATIVES To gain insight as to how the pigments inactivate the preservatives, the adsorption of preservatives on pigments was measured by HPLC (Table II). The adsorption of MP on these pigments was small, compared to that of EP. This result is inversely related to the inactivation of p-hydroxybenzoic acid esters by these pigments. Further investigation was done to determine whether such a decrease of MP affected the bactericidal activity or not. In the presence of ultramarine blue, solubilized MP was decreased from 0.2% to 0. 198%, which means 0.002% of MP was adsorbed on the ultramarine blue. This decrease of the solubilized MP did not show any effect on the bactericidal activity toward S. aureus (Table III). Thus, from this result, the inactivation MP EP PP BP Lot No.K-842 Lot No.1243 Lot No.1234 .... ...........-.., -,•....-..-.:,., .. '.••• ::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: i ! ! i ! i 10 10-3 10 -1 I.D. Figure 4. Effects of ultramarine blue on the bactericidal activity of p-hydroxybenzoic acid esters against Staphylococcus aureus.