86 JOURNAL OF COSMETIC SCIENCE 0.7 0.6 0.5 0.4• 0.3' 0.2- 0.1-' 0.0- -0.1 Untreated hair z• = CuUcle - --o-- Resin A./ 36'00 35'00 34'00 '33'00 32'00' Frequency (crn '•) Bleached hair 0.7 0.6 0.5 0.4 0.3 0.2 0.1 0.0 -0.1 Resin Cuticle x N 3600 3500 3400 3300 3200 Frequency (cm '•) Figure 11. Infrared spectra between 3102 cm ] and 3700 cm •of the cuticle and cortex and the embedding resin of the untreated hair (A) and the hair after bleaching (B). Recording conditions are the same as in Figure 8. The ratio A/B relative to the peak height A and B with the baseline MN was calculated to quantify the water amount (shoulder centered at 3400 cm -z) in the samples.

CHEMICAL IMAGING OF HAIR BY IMS 87 A: Chemical Image of the of the cuticle and cortex 2O 15 i o o lO 15 20 25 30 x Axis (microns) Absorbance gvCH band' 2875 cm 4) [• resin I cuticle I cortex B' Chemical image of the Bonded water (grey color) in the area of the cuticle 2O 15 B/A [• 0.85 0.85-1.1 ll.l 0 5 10 15 2o 25 3o x Axis (microns) Figure 12. Chemical images of the edge of t he bleached hair. Recording conditions are the same as in Figure 5B. A: Visualization of the cuticle and cortex: absorbance contour map for the vCH band (2875 cm-•). B: Distribution of the bonded water in the same area (B/A ratio: see Figure 10).