212 JOURNAL OF THE SOCIETY OF COSMETIC CHEMISTS (12) H. W. Gibson, J. M. Pochan, and F. C. Bailey, "Surface analyses by a triboelectric charging tech- nique, A,al. Chem., 51(4), 483 (1979). (13) H. W. Gibson, Control of electrical properties of polymers by chemical modification, Polymer, 25, 3 (1984). (14) H. W. Gibson, Linear free energy relationships. V. Triboelectric charging of organic solids, J. Am. Chem. Soc., 97, 3832 (1975). (15) H. W. Gibson, "Dye Sulfonated Polystyrene Films: Relationship of Triboelectric Charging and Molecular Orbital Energy Levels," in ModijS'cation of Polymers, Ch. E. Carraher, Jr., and J. A. Moore, Eds. (Plenum Publ. Co., 1983), p 353. (16) J. Lowell, The relationship between contact charging and the concentration of donor impurities in polymers, J. Phys. D..' Appl. Phys., 12, 2217 (1979). (17) C. Barnes, P. J. Bower, and T. J. Lewis, Electrical charge transfer at metalbiopolymer interfaces, Annual Report, Conference on Electrical Insulation and Dielectric Phenomena (IEEE Electrical Insulation Soc., 1982), p 87. (18) J. P. Crine, Open-circuit voltages and their influences on the dielectric properties of metal-dielectric- metal systems, Annual Report, Conference on Electrical Insulation and Dielectric Phenomena (IEEE Electrical Insulation Soc., 1984), p 474. (19) J. Jachowicz, G. Wis-Surel, and L. J. Wolfram, Directional triboelectric effect in keratin fibers, Text. Res. J., 54(7), 492 (1984). (20) A. J.P. Martin, Triboelectricity in wool and hair, Proc. Phys. Soc., London, 53(2), 186 (1940). (21) A. C. Lunn and R. E. Evans, The electrostatic properties of human hair, J. Soc. Cosmet. Chem., 28, 549 (1977). (22) P. Dubin and I. Levy, GPC of cationic polymers on PW gel columns, J. Chromatogr., 235, 377 (1982). (23) D. A. Seanor, Techniques for the prevention and elimination of static from polymer coatings, Polymer- Plast, Technol. Eng., 3(1), 69 (1974). (24) C. B. Duke, T. J. Fabish, and A. Paton, Influence of polarization fluctuations on the electronic structure of molecular solids, Chem. Phys. Lett., 49(1), 133 (1977). (25) G. Bush and H. Schade, Lectures on Solid State Physics (Pergamon Press, New York, 1976), p 489. (26) C. B. Duke and T. J. Fabish, Charge-induced relaxation in polymers, Phys. Rev. Lett., 37(16), 1075 (1976). (27) G. Bush and H. Schade, Lectures on Solid State Physics (Pergamon Press, New York, 1976), p 489. (28) The Handbook of Chemistry and Physics, 57th Edition, CRC Press, 1976-77. (29) P. K. Watson, "The Transport of Electrons From the Surface Into the Bulk of Polystyrene," in Polymer Surfaces, D. T. Clark and W. J. Feast, Eds. (John Wiley and Sons, Chichister, 1978), p 91. (30) P. Finkelstein and K. Laden, The mechanism of conditioning of hair with alkyl quaternary ammonium compounds, Appl. Polym. Syrup., 18, 673 (1971). (31) A. G. deBoos and E. D. Finnimore, The determination of ionic surfactants on wool, Tenside Detergents, 19, 262 (1982). (32) C. V. Patel, Antistatic properties of some cationic polymers used in hair care products, Int. J. Cosmet. Sci., 5(5), 181 (1983). (33) J. Jachowicz, G. Wis-Surel, and M. Garcia, Relationship between triboelectric charging and surface modification of human hair. Polymeric versus monomeric long alkyl chain quaternary ammonium salts, in preparation. (34) J. Jachowicz, M. Berthiaume, and M. Garcia, The effect of the amphiprotic nature of human hair keratin on the adsorption of high charge density cationic polyelectrolytes, submitted to Coll. Polym. Sci.

j. Soc. Cosmet. Chem., 36, 213-222 (May/June 1985) Quantitative analysis of N-nitrosodiethanolamine (NDELA) by high performance liquid chromatography-thermal energy analyzer detection MITCHELL D. ERICKSON, DUANE B. LAKINGS, ARBOR D. DRINKWINE, and JAMES L. SPIGARELLI, Midwest Research Institute, 425 Volker Boulevard, Kansas City, MO 64110. Received December 1 O, 1984. Presented in part at the Pittsburgh Conj•rence on Analytical Chemistry and Applied Spectroscopy, Atlantic City, N J, March 10-14, 1980. Synopsis A method for the analysis of N-nitrosodiethanolamine using high performance liquid chromatography with thermal energy analyzer detection (HPLC-TEA) is presented. Under the normal phase HPLC con- ditions required by the TEA, optimum chromatography was achieved using 75/19/6 (v/v/v) isooctane/ methylene chloride/methanol mobile phase on a Spherisorb CN column. The use of an internal chromato- graphic standard, N-nitrosopyrrolidine, was shown to double the quantitative precision. INTRODUCTION N-nitrosamines have long been recognized as a group of hazardous compounds about 80% of these tested have been found to be carcinogenic to test animals (1-4). Their carcinogenicity, mutagenicity, health effects, environmental presence, and occurrence in commercial products have been reviewed (1-10). In 1977, it was reported that N- nitrosodiethanolamine (NDELA) had been found in cutting fluids (11), tobacco (12), cosmetics (13), lotions (13), and shampoos (13). Subsequently, NDELA was shown to be a carcinogen in the rat (14). Since these reports, scientists in industry and govern- ment have been working to develop and improve upon analytical methods for detecting NDELA (15). Although there have been some efforts directed at NDELA analysis by gas chromatog- raphy/flame ionization detection of derivatives (16), most methods have employed high performance liquid chromatography (HPLC) with UV (17-20) or thermal energy an- alyzer (TEA) (13,21) detection. HPLC-TEA provides not only the selectivity of an N- nitrosamine specific detector, but also low detection limits (1-5 ng for many N- nitrosamines). The use of UV irradiation to destroy N-nitrosamines as a confirmatory technique has been previously reported (22). The N-NO moiety undergoes photolyric cleavage, de- stroying the nitrosamine. The disappearance of a TEA peak following UV irradiation therefore adds confidence to the identification. 213