490 50% - � � 40% rlJ = � • 30% ·- = c 20% ·- 0% -10% JOURNAL OF COSMETIC SCIENCE Eucalyptus ----1 ► �� Pa ·cl o Figure 7. Improvement in the luster of che hair shaft after application of Eucalyptus extract lotion (3wt%) for three months (Group 2). This includes the results from panelists using the placebo lotion. Table II Comments of Panelists in Group 2 after Using Eucalyptus Extract Scalp Lotion for Three Months Panelists' initials SN NM FdK TC AM TM TR OK FsK YS IK Comments Hair root made strong, not oily, not dry. Scalp is healthy. Hair root made more shiny. More hair growth and stronger hair. No hair loss. I feel the volume on the top of the head. Hair roots feel soft and smooth. Scalp is not dry. My hair made thicker, with more body. Easy-to-make hair style. Hair made smooth and feels good. My hair made thicker but smooth. Scalp too. Hair root is soft and shiny. My hair made strong. No comment. No comment. Hair loss decreased. My hair returned to health. Hair loss decreased because number of hairs removed with my hairbrush decreased after using this lotion. Itching of my scalp stopped after using chis lotion. The difference in microstructure of the entire hair could not be recognized by X-ray measurement such as SAXS and WAXD (Figure l0a,b). As Young's modulus changes had been confirmed from the nano-indentation analytical result by AFM, we had to look for a spatially selective technique for the measurement of the cortex and the analysis of the secondary structure of protein. In the case of the selective measurement of the cortex by X-ray, large and specialized equipment and facilities, such as synchrotron radiation, are needed.

12.0 _ 11.s � ,:a.. c., ';' 11.0 = "CS 10.5 = fl.l --r 10.0 = = � 9.5 9.0 EUCALYPTUS EXTRACT-INDUCED HAIR CHANGES p=0.0118 - fl.l 1/ ·-..., = t � ..., :=---=== • ;Ei _________. - ·-�c fl.l � = fl.l fl.l = c., ·- � = 0 months 2 months 300 250 200 150 100 p=0.0029 I I .� ____________. 0 months 2 months 491 --1 ! I I i Figure 8. Bending stiffness and hair gloss intensity of Caucasian panelists (Group 4) before and after using 3% Eucalyptus extract scalp lotion for two months. *:p0.05 Panelist S PanelistM **:p0.01 t-test (N=23) (N=23) 420 ** 680 � * � Q, 400 ** Q, 640 ** � 380 ** 600 "O "O ** 0 0 � 360 � 560 -bO -bO d d = 340 g 520 0 - - 320 480 Placebo Eucalyptus Placebo Eucalyptus Figure 9. Results of nano-Indentation analysis in hair cortex by AFM. Left side: panelist S. Right side: panelist M. Two pieces of new growth of hair from Eucalyptus extract lotion-treated and placebo side, in each panelist, respectively. Nano-indentation was carried out at 25 points on each hair. The highest and lowest data were neglected at !-test. Recently, microscopic IR has become a powerful and useful analytical method for studying biological samples, since the appearance of FT-IR could shorten the measuring time dramatically. The microscopic IR spectrum method is sufficient for the selective measurement of the cortex in the cross section of the human hair, although the spatial resolution of 4 micrometer is inferior to the Raman spectrum using a microscopic method. Tertiary structural analysis becomes possible by the measurement of XRD and NMR, while IR spectra provide the secondary structure of the protein. IR spectra being adequate enough for the analysis of the secondary protein structure, in order to consider the relationship between the change in the physical properties of the hair and the