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]. Cosmet. Sci.J 57, 279-289 CTuly/August 2006) Inhibitory effects of antioxidant constituents from Melathria heteraphylla on matrix metalloproteinase-1 expression in UVA-irradiated human dermal fibroblasts Y. H. CHO, J. H. KIM, G. S. SIM, B. C. LEE, H. B. PYO, and H. D. PARK, R&D Center, Hanbul Cosmetics Corporation, 72-7, Yongsung-Ri, Samsung-Myun, Umsung-Kun, Chungbuk 369-834 (Y.H.C., ].H.K., G.S.S., B.C.L., H.B.P.), and Department of Biotechnology, College of Engineering, Daegu UniversityJ Gyeongsan 712-714 (H.D.P.), Korea. Accepted for publication February 21, 2006. Synopsis Matrix metalloproteinases ( MMPs) are known to play an important role in photoaging by mediating the degradation of extracellular matrix proteins. To develop a new anti-aging agent for cosmetics from natural products, Melothria heterophylla (Lour.) Cogn. was selected for its antioxidant activity and inhibitory effect on expression of MMP-1 in UVA-irradiated human skin fibroblasts. Two compounds (compounds 1 and 2) were isolated from an ethyl acetate soluble fraction of the ethanolic extracts they were identified as l ,2,4,6-tetra-0-galloyl-�-0-glucopyranose (1) and 3,4,5-trihydroxybenzoic acid (2). These compounds were found to scavenge radicals and reactive oxygen species (ROS) and were measured to have SC 50 values of 3.9 µMand 13.3 µM against 1,1-diphenyl-2-picrylhydrazyl (DP PH) radicals and values of 4.3 µMand 4.0 µM against superoxide radicals in the xanthine/xanthine oxidase system, respectively. Compounds 1 and 2 showed a dose-dependent inhibitory effect on the expression and activity of MMP-1 in UV A-induced human skin fibroblasts, but no inhibition of MMP-1 mRNA expression. Therefore, we concluded that compounds 1 and 2 significantly inhibited MMP-1 expression at the protein level. Also, these compounds were determined to have a potent antioxidant activity. From these results, we suggest that these compounds might be useful as a new anti-aging agent for photodamaged skin, but the in vitro findings must be verified in in vivo studies. INTRODUCTION The skin is increasingly exposed to ambient UV irradiation, thus increasing risks for photooxidative damage with long-term detrimental effects like photoaging, character­ ized by wrinkles and loss of skin tone and resilience (1). UV irradiation is known to produce reactive oxygen species (ROS), which initiate the degradation of the extracel- Address all correspondence to Y. H. Cho. 279