Table I Dyes Tested Colour index no. Chemical Induction cone. Challenge cone. in propylene glycol2 Colour index name (other names) 1 or chemical abstract no. class in propylene glycol 10.00% 5.00% 2.50% 2-Amino-4-ni trophenol 99-57-0 Nitroaminophenol 10.00% + (80%) + (70%) + (40%) u 2-Amino-5-nitrophenol 121-88-0 Nitroaminophenol 10.00% + (30%) + (10%) Negative Acid black 2 50420 Azine 10.00% Negative Negative Negative tTJ Acid black 24 26370 Azo 10.00% Negative Negative Negative Acid blue 1 (Food blue 3) 42045 Tri pheny !methane 10.00% Negative Negative Negative z Acid blue 45 63010 Anthraquinone 10.00% Negative Negative Negative d Acid brown 359 Not assigned Monoazo 10.00% Negative Negative Negative n Acid yellow 23 (Tartrazine/yellow 5) 19140 Monoazo 10.00% + (20%) Negative Negative tTJ Acid orange 3 10385 Nitro 10.00% + (80%) + (60%) + (30%) d Acid red 50 45220 Xanthene 10.00% Negative Negative Negative Basic black 3 11825 Monoazo 10.00% + (100%) + (100%) + (50%) r Basic orange 1 11320 Monoazo 10.00% + (100%) + (80%) + (70%) tTJ Basic red 2 50240 Azine 10.00% Negative Negative Negative � Basic violet 1 (Gentian violet) 42535 Triarylmethane 10.00% Negative Negative Negative - Basic violet 2 42520 T riary !methane 10.00% Negative Negative Negative n Basic violet 3 (Gentian violet) 42555 Triarylmethane 10.00% Negative Negative Negative n Basic violet 13 42536 T riary !methane 10.00% Negative Negative Negative 0 Disperse black 9 12222-69-4 Azo 10.00% Negative Negative Negative z Disperse blue 1 (Solvent blue 18) 64500 Anthraquinone 10.00% Negative Negative Negative Disperse orange 3 (Solvent orange 9) 11005 Monoazo 10.00% + (50%) + (30%) Negative n Disperse red 5 5 Not assigned Anthraquinone 10.00% Negative Negative Negative """"1 Disperse violet 4 (Solvent violet 12) 61105 Anthraquinone 10.00% Negative Negative Negative d Disperse violet 11 Not assigned Anthraquinone 10.00% Negative Negative Negative tTJ Disperse yellow 49 Not assigned Methine 10.00% Negative Negative Negative � Disperse yellow 232 55165 Azo 10.00% Negative Negative Negative HC red 3 1/4/2871 Nitrophenylenediamine 10.00% Negative Negative Negative """"1 HC yellow 4 59820-43-8 Ni troaminophenol 10.00% Negative Negative Negative - HC yellow 5 56932-44-6 Nitrophenylenediamine 10.00% Negative Negative Negative Solvent black 5 50415 Azine 10.00% Negative Negative Negative C/'J Solvent black 27 Not assigned Not assigned 10.00% + (70%) Negative Negative Solvent black 34 12195 Monoazo 10.00% + (100%) Negative Negative Solvent blue 6 44040:1 T riary !methane 10.00% Negative Negative Negative Solvent red 122 Not assigned Azo 10.00% Negative Negative Negative N 1 Information pertaining to Colour Index nos. and chemical class obtained from Colour Index (17). ...... 2 Results expressed as negative or positive ( +) with the percent of animals in the group demonstrating an allergic reaction in parentheses.

Table II Dose Response of Dyes Eliciting Positive Reactions at the 10% Level Colour index no. Chemical Induction cone. Challenge cone. in propylene glycoi2 Colour index name (other names) 1 or chemical abstract no. class in propylene glycol 1.00% 0.50% 0.25% 2-Amino-4-ni trophenol 99-5 7-0 Nitroaminophenol 1.00% + (30%) Negative Negative 2-Amino-5-nitrophenol 121-88-0 Ni troaminophenol 1.00% + (40%) + (20%) + (10%) Acid yellow 23 (Tartrazine/yellow 5) 19140 Monoazo 1.00% + (10%) Negative Negative Acid orange 3 10385 Nitro 1.00% Negative Negative Negative Basic black 3 11825 Monoazo Not tested Not tested Basic orange 1 11005 Monoazo 1.00% Negative Negative Negative Disperse orange 3 (Solvent orange 9) 11005 Monoazo 1.00% + (10%) Negative Negative Solvent black 27 Not assigned Not assigned 1.00% Negative Negative Negative Solvent black 34 12195 Monoazo 1.00% + (20%) + (20%) Negative 1 Information pertaining to Colour Index nos. and chemical class obtained from Colour Index (17). 2 Results expressed as negative or positive ( +) with the percent of animals in the group demonstrating an allergic reaction in parentheses. N """"' N 0 C � 0 "Tj n 0 tT:l f--1 - n er, n - tT:l z n tT:l