DETERMINATION OF SUNSCREEN AGENTS BY UPLC AND HPLC 479 Table V Theoretical Concentrations (TC), Observed Concentrations (OC), and Recovery for Accuracy Studies by HPLC and UPLC Sunscreen TC oc Recovery agent Instrument (µg ml- 1 ) (µg ml- 1 ) (%) IMC HPLC 43.22/47 .58/5 7 .19 42.88/48.19/57 .57 99.20/101.28/100.67 UPLC 42.99/48.15/5 7 .63 99.47 /101.20/100.77 OMC HPLC 130.97 /143.13/170.97 130.35/145.44/172.57 99.5 3/101.61/100.98 UPLC 130.45/141.96/172.33 99 .60/99.18/100.80 OS HPLC 95.26/108.31/130.35 96.36/109.90/131.97 101.15/101.47 /101.24 UPLC 95 .84/109.18/131.25 100.61/100.80/100.69 OT HPLC 41.5 7 /47 .12/57.45 41.15/47 .57 /5 7. 74 99.00/100.96/100.51 UPLC 41.99/47.95/57.34 101.08/101. 76/99.81 TM HPLC 22.81/25 .23/29. 78 23.13/25.50/29.89 101.64/101.07 /100.38 UPLC 22.93/25.10/29.94 100.5 3/99.48/100.54 TS HPLC 31.28/34.21/41.41 31.05/34.77 /45.54 99.26/101.64/100.32 UPLC 31.33/34.56/41.3l 100.16/101.02/99.76 using the proposed HPLC and UPLC methods in this study. The test data corresponded exactly with the indicated ingredients of the cosmetics. The results are tabulated in Table VI. Table VI Determination of Sunscreen Agents in Suncare Products by HPLC and UPLC Label claim % Found % Found Product UV filter (%, w/w) in HPLC* in UPLC* Cream A IMC 2.0 2.1 2.1 OMC 5.0 5.3 5.3 OS 4.5 4.7 4.7 OT 2.0 2.2 2.2 Cream B IMC 2.0 2.0 2.0 OMC 5.7 5.8 5.7 TM 1.0 1.1 1.0 TS 2.0 2.0 2.0 Lotion A IMC 2.0 2.1 2.1 OMC 7.0 7.2 7.1 Lotion B IMC 2.0 2.2 2.1 OMC 5.0 5.1 5.1 TM 1.0 1.1 1.1 TS 2.0 2.0 1.9 Foundation A IMC 2.0 2.1 2.0 OMC 5.0 5.0 5.0 TS 3.0 3.2 3.1 Foundation B OMC 3.0 3.2 3.2 TS 6.0 6.4 6.2 Makeup base A IMC 3.0 3.1 3.1 Makeup base B IMC 2.0 2.1 3.1 OMC 7.0 7.2 7.0 OS 4.5 4.6 4.5 TS 3.0 3.1 3.1 Lipstick A IMC 2.0 1.9 2.0 OMC 5.0 5.0 5.1 Lipstick B OMC 7.0 7.3 7.2 * Values are the average of 3x determination.

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