EUCALYPTUS EXTRACT-INDUCED HAIR CHANGES 487 Eucalyptus Placebo (a) Stand up from the root part (b) Narrow Wide Figure 3. Example of the improvement in hair shaft properties after one month of continuous application of Eucalyptus extract lotion. (a) Increased stand-up with EL. (b) Left side of panelist's head, treated with Eucal ptus extract lotion (3wt%) right side of panelist's head, treated with placebo lotion. Table I Comments of the Panelists in Group l about the Side Treated with Eucalyptus Extract Lotion after Three Months of Use Panelists' initials IJ NG FJ KI MY YR KT SK SG ST MA Comments My hair root srood up, so my parring became narrow. After using this scalp lotion, my hair was not flattened even in high humidity. My scalp became silky smooth. I aguired my hair volume again. Also, hair luster increased after usage. After natural drying, fringe did not part. It's enough ro run a brush through my hair. I was not aware of my scalp, because I could not see it. I got hair volume! My hair became healthy and smooth. My hair became easier to handle. Hair loss decreased after two months of use. Scalp became soft. Hair gloss increased after using this lotion. Hair loss decreased. panelists (Group 2), although the values showed some fluctuations. Table II summarizes the results of the interview with the EL panelists after three months of use. More than 70% of the panelists had a positive perception about the change in the physical prop­ erties of hairs after using the Eucalyptus extract lotion.

488 JOURNAL OF COSMETIC SCIENCE � rlJ 200 ....... ·-= p0.01 = 180 = - ....... � 160 = '-"' � ·- 140 rlJ = � 120 rlJ rlJ Q -- C, 100 - =·- 80 Placebo Eucalyptus Figure 4. Hair gloss intensity after three months of half-head use test of continuous application of Eucalyptus extract lotion and placebo lotion (Group 1). Figure 8 displays the results for Caucasian panelists who live in Japan (Group 4), after two months of use of 3% EL. The Caucasian panelists also confirmed the perception of increases in hair bending stiffness and hair gloss intensity using the 3% Eucalyptus extract. As described above, Eucalyptus extract has an effect on improving hair properties, regardless of gender difference, age, and race. ANALYSIS BY ATOMIC FORCE MICROSCOPE (AFM) In order to analyze the improvement factor of the bending elasticity, Young's modulus at the new-growth part of the cortex of the hair treated with Eucalyptus extract and the placebo was measured by AFM. A flat and smooth hair section was made in epoxy resin by ultramicrotome and the AFM nano-indentation measurement was carried out. The measurement data were analyzed by the Hertz model according to the method of Parbhu (25). The calculation results indicated that the Young's modulus of the Eucalyptus extract-treated hair was significantly higher than that of the placebo-treated hair in all samples from two panelists (Figure 9). ANALYSIS BY MICROSCOPIC IR SPECTRUM METHOD Secondary structural analysis of the hair cortex was carried out to test whether the