EUCALYPTUS EXTRACT-INDUCED HAIR CHANGES 489 Eucalyptus Extract 0.5wt% Eucalyptus Extract 1.0wt¾ Eucalyptus Extract 3.0wt¾ 12.0 -------�-------- 12.0 12.0 I'll = "'a 11.0 I'll "'� 10.5 = 10.0 9.5 Placebo Eucalyptus 11.5 11.5 11.0 11.0 10.5 10.5 10.0 ! 10.0 9.5 9.5 Placebo Eucalyptus Placebo Eucalyptus Figure 5. Results of 2.5 months of half-head use tests of scalp lotions containing 0.5%, 1 %, and 3% Eucalyptus extract lotion vs placebo lotion (Group 3 ). 30% - ·- == c.. Q 208/o rll rll Q-' J9 ·- r:I.J. = ·- Q-' rll = Q-' - y = Iii-I 10% 0% -10% l .. .___ ________________________________J Figure 6. Change in the bending stiffness of the hair shaft by application of Eucalyptus extract lotion (3wt%) for three months (Group 2). This includes the results from panelists using the placebo lotion. changes in gloss and Young's modulus at the new-growth part of the hair treated with Eucalyptus extract were related to changes in the hair microstructure. Solid-NMR, CD, XRD, and IR had been considered for analysis of the secondary structure of the hair protein. Solid-NMR could not be applied, since we could not prepare enough samples for measurement by solid-NMR from this survey (26). Since the sample condition should be a solution state in the case of CD measurement, preprocessing is needed for the analysis of the secondary protein structure of the hair (27). Such preprocessing changes the secondary structure of hair protein. For this reason, CD measurement was not used, in order to avoid the denaturation of protein.

490 50% - � � 40% rlJ = � • 30% ·- = c 20% ·- 0% -10% JOURNAL OF COSMETIC SCIENCE Eucalyptus ----1 ► �� Pa ·cl o Figure 7. Improvement in the luster of che hair shaft after application of Eucalyptus extract lotion (3wt%) for three months (Group 2). This includes the results from panelists using the placebo lotion. Table II Comments of Panelists in Group 2 after Using Eucalyptus Extract Scalp Lotion for Three Months Panelists' initials SN NM FdK TC AM TM TR OK FsK YS IK Comments Hair root made strong, not oily, not dry. Scalp is healthy. Hair root made more shiny. More hair growth and stronger hair. No hair loss. I feel the volume on the top of the head. Hair roots feel soft and smooth. Scalp is not dry. My hair made thicker, with more body. Easy-to-make hair style. Hair made smooth and feels good. My hair made thicker but smooth. Scalp too. Hair root is soft and shiny. My hair made strong. No comment. No comment. Hair loss decreased. My hair returned to health. Hair loss decreased because number of hairs removed with my hairbrush decreased after using this lotion. Itching of my scalp stopped after using chis lotion. The difference in microstructure of the entire hair could not be recognized by X-ray measurement such as SAXS and WAXD (Figure l0a,b). As Young's modulus changes had been confirmed from the nano-indentation analytical result by AFM, we had to look for a spatially selective technique for the measurement of the cortex and the analysis of the secondary structure of protein. In the case of the selective measurement of the cortex by X-ray, large and specialized equipment and facilities, such as synchrotron radiation, are needed.