398 JOURNAL OF COSMETIC SCIENCE Gender and age differences in the numbers and frequencies of melanocytes and melanoblasts in the skin treated with MEL/NB-UVB alone and MEL/NB-UVB +MTK are shown in Table III. No significant differences were observed between them. COLLAGEN AND ELASTIN FIBERS In the skin of patient 41, collagen fibers were well developed in nonlesional (Figure 3A), lesional (Figure 3B), MEL/NB-UVB-treated (Figure 3C), and (MEL/NB-UVB +MTK)-treated (Figure 3D) skin. No marked differences were observed in the distribution and density of collagen fibers. Figure 2. Histochemical sections of patients 44 (A, B, C, D) and 51 (E, F, G, H) are shown. The DOPA reaction detecting melanocytes (M) of nonlesional skin (A, E) revealed many melanocytes with dendrites (arrows). Although melanocytes were also present in (MEL/NB-UVB +MTK)–treated skin (B, F), epidermal melanin pigmentation was much less than that of nonlesional skin (A, E). The combined DOPA–premelanin reaction (C, D, G, H, M +Mb) revealed dark-stained melanocytes and lightly-stained melanoblasts (Mb). Melanoblasts (short arrows) were lightly stained, while melanocytes (long arrows) were darkly stained. Scale bar =25 µm. Original magnification ×400.

Table III Gender and Age Differences in the Melanocyte and Melanoblast Populations/0.1 mm2Interfollicular Epidermis of the Skin Treated With MEL/NB-UVB Alone and MEL/NB-UVB +MTK Freq/Cell no MEL/NB-UVB, MTK No. of melanocytes/0.1 mm2 No. of melanoblasts/0.1 mm2 (A)Gender Female Male Female Male Freq MEL/NB-UVB alone 0% (0/6) 9.1% (1/11) 0% (0/6) 9.1% (1/11) MEL/NB-UVB +MTK 55.6% (5/9) 62.5% (5/8) 55.6% (5/9) 62.5% (5/8) Cell no MEL/NB-UVB alone 0 (N =6) 0.22 ± 0.22 (N =11) 0 (N =6) 0.46 ± 0.46 (N =11) MEL/NB-UVB +MTK 19.7 ± 11.2 (N =9) 32.9 ± 12.3 (N =8) 22.2 ± 10.4 (N =9) 33.5 ± 12.5 (N =8) (B)Age Young Old Young Old Freq MEL/NB-UVB alone 12.5% (1/8) 0% (0/9) 12.5% (1/8) 0% (0/9) MEL/NB-UVB +MTK 62.5% (5/8) 55.6% (5/9) 62.5% (5/8) 55.6% (5/9) Cell no MEL/NB-UVB alone 0 (N =8) 0.27 ± 0.27 (N =9) 0 (N =8) 0.57 ± 0.57 (N =9) MEL/NB-UVB +MTK 30.0 ± 12.7 (N =8) 22.3 ± 11.1 (N =9) 36.4 ± 12.5 (N =8) 19.6 ± 10.0 (N =9) Freq, frequency of differentiation of melanocytes/melanoblasts after the treatment with MEL/NB-UVB alone and MEL/NB-UVB +MTK. MTK, treatment with FFC, collagen peptide, elastin peptide, and UCB-MSCCS. Young patient, from 4 to 30 years Old patient, from 31 to 76 years. Each datum of cell number (Cell no) is an average ± standard error of the mean. No significant differences in gender and age were observed. 399 MESENCHYMAL STEM CELL-DERIVED FACTORS