FRAGRANCE COMPLEXITY, FAMILIARITY, AND PLEASANTNESS 97 REFERENCES (1) J. Stephan Jellinek and Egon P. K•Sster, Perceived fragrance complexity and its relation to familiarity and pleasantness,.]. Soc. Cosmet. Chem., 30, 253-262 (1979). (2) J. StephanJellinek, Evaporation and the odor quality of perfumes,.]. Soc. Cosmet. Chem. 12, 168 (1961).

J. Soc. Cosmet. Chem., 34, 99-:[25 (March/April 1983) Allergens of lanolin: parts I and II SATOSHI TAKANO, MAKOTO YAMANAKA, KIKUHIKO OKAMOTO, Tochigi Research Laboratories, KAO Corporation, 2606, Akabane, Ichikai- machL Tochigi 321- 34, Japan FUMIO SAITO, Department of Dermatology, Faculty of Medicine, Toho University, 2-17-6, Ohashi, Megro-ku, Tokyo 153, Japan. Received February 19, 1982. PART I: ISOLATION AND IDENTIFICATION OF THE ALLERGENS OF HYDROGENATED LANOLIN Synopsis Isolation and identification of the allergens of hydrogenated lanolin were investigated. Hydrogenated lanolin was fractionated with column chromatography on Florisil. A fraction which indicated positive allergic reaction on hydrogenated lanolin allergy patients was further fractionated with TLC on alumina. Components of the subdivided allergenic fractions were precisely identified by means of GC-MS. It was found that the main aliphatic components of the allergenic fraction were alkane-c•,/3-diols and alkane-c•,c0-diols. Therefore, these diols were isolated from hydrogenated lanolin by the use of column chromatography, urea adduct method, and high performance liquid chromatography. Because most patients showed strong allergic reactions to these isolated diols, it was confirmed that these were major allergenic components of hydrogenated lanolin. INTRODUCTION Because of its particular physical and chemical properties, wool grease or its purified product, lanolin, obtained from wool fat, is extensively used in the industrial fields as a metal lubricant, rust preventive agent, fiber treatment agent, and lustering agent. Besides the industrial applications, it is widely utilized in pharmaceutical preparations and cosmetics. The effectiveness of lanolin as a cosmetic material results not only from its emulsive, dispersive, and solubilizing properties, but also from its role as a raw material for the production of dermatologically and cosmetically ideal oils or fats which resemble human skin surface lipids (1). To meet the widespread demand, lanolin is subjected to physical or chemical .... treatment. In the field of cosmetics, it is utilized as a cosmetic base in liquid, hard, and hydrogenated forms. Lanolin and its derivatives have long been used, but their composition is not