M. HETEROPHYLLA AND INHIBITION OF MMP-1 287 (A) 1 2 3 4 5 MMP-1 237 bp �-actin ------- 248 bp (B) 140 * 120 Q) 100 � - 80 0 z C: 0:: 0 60 E CJ - Q) 0 � 40 � Q) 0:: 20 0 Non-UVA UVA tRA Comp. 1 Comp. 2 Figure 4. Effects of compounds 1 and 2 isolated from M. heterophylla on MMP-1 mRNA level in UVA­ irradiated human dermal fibroblasts. (A) After irradiation by UVA, HDFs were treated with various concentrations of these compounds for 12 h. Total RNA extracted from HDFs was analyzed by RT-PCR, and each band, which is quantirated by a densitometer using 13-actin transcripts as references, in (A) corresponds to each bar in (B). Lane 1: UV A non-irradiated. Lane 2: UV A (6.3 J/cm2 ) irradiated. Lane 3: UV A + 4.0 µM of tRA. Lane 4: UV A + 6.0 µM of compound 1. Lane 5: UV A + 30.0 µM of compound 2. CONCLUSIONS Two compounds isolated from the roots of M. heterophylla exhibited significant DPPH and superoxide anion radical scavenging activities. Also, these compounds significantly reduced the activity and expression of MMP-1 at the protein level in a dose-dependent manner. Therefore, we suggest that these compounds may be potential candidates to protect the skin from the aging effects of UV stimulation. REFERENCES (1) M. Wlaschek, I. Tantcheva-Poor, L. Naderi, W. Ma, L.A. Schneider, Z. Razi-Wolf, J. Schuller, and K. Scharffetter-Kochanek, Solar UV irradiation and dermal photoaging, J. Photochem. Photobiol., 63, 41-51 (2001).

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