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J. Cosmet. Sci.J 59, 441-448 (September/October 2008) Simultaneous determination of heavy metals in cosmetic products SO-MI LEE, HYE-JIN JEONG, and 1H SEOP CHANG, R&D Center, Amore Pacific Corporation} 314-1 Bora-dong, Giheung-gu, Yongin-si, Gyeonggi-do, 446-7 29, Republic of Korea. Accepted for Publication April 9, 2008. Synopsis An extremely small amount of several heavy metals have been detected in cosmetic products as impurities, which can cause skin allergies through percutaneous adsorption on the skin. We present here a fast, accurate, and highly sensitive method for simultaneous determination of Pb2 + , Fe2+, Cu2+, Ni2+, Zn2 + , Co2 + , Cd2 + and Mn2 + in coloring agents and cosmetic products, to be evaluated by ion chromatography. All of these metals are well separated through a bifunctional ion-exchange column (IonPac CSSA) and detected by post-column reaction and spectrophotometric detection. The calibration graphs are linear (r2 0.999), in the range 0.1-1000 µg/ml. Detection limits for a 200-µl sample solution are at the µg/1 level, which is sufficient for judging whether the product is safe or not. The relative standard deviations (RSDs) of the retention time and the peak area are less than 0.21 % and 1.24%, respectively. The recovery rates are 97-104%. The result shows that the proposed determination method is more sensitive, more accurate, and faster than current methods such as HPLC, ICP-MS and Flame-AAS. The new method was applied to analyze the amount of heavy metals contained in 22 cosmetic products and 11 coloring agents. INTRODUCTION Many cosmetic products contain heavy metals such as lead, arsenic, mercury, cobalt, and nickel as ingredients or impurities. Recent research has reported that these metals can easily cause many types of skin problems (1,2). The use of some heavy metals in cosmetics has been controversial due to the biological accumulation of those metals and their toxicity in the human body. In most countries, it is legally prohibited to use lead, arsenic, and mercury in skin cosmetic products. For example, lead is prohibited as part of cosmetic compositions in Korea, the European Union, and China. The maximum allowable level of lead is 20 ppm in those jurisdictions. There are no specific rules on other heavy metals, such as cobalt, nickel, and copper (3 ). It is also reported that those metals can cause allergic contact dermatitis or other skin problems (4,5). To provide customers with safe cosmetics, it is Address all correspondence to So-Mi Lee. 441