466 JOURNAL OF COSMETIC SCIENCE mAU 350 I.S. 160 100 0 H '15 min Figure 6. Chromatogram obtained after injecting 20 µl of an extract deriving from commercial sample 5, recorded at 333 nm. Waiting for the decisions of the European Committee concerning possible restrictions for the use of vitamins K 1 and K3 in the cosmetic field, we can conclude that the developed analytical procedure is suitable to be used by laboratories for routine control in order to help the national authorities in their surveillance activity and to better understand if the reported sensitization effects are related to the amounts of these vitamins present in the cosmetic samples. ACKNOWLEDGMENTS The authors thank Mr Gianluigi Pinci from Istituto Superiore di Sanita for his helpful support in computer science, and Mr Antonio Parlato for his helpful assistance. REFERENCES (1) M. Kamao, Y. Suhara, N. Tsugawa, and T. Okano, Determination of plasma vitamin K by high performance liquid chromatography with fluorescence detection using vitamin K analogs as internal standards,]. Chromatogr. B, 816, 41--48 (2005). (2) D. J. Harrington, R. Soper, C. Edwards, G. F. Savidge, S. J. Hodges, and M. J. Shearer, Determination of the urinary aglycone metabolites of vitamin K by HPLC with redox-mode electrochemical detec tion,] Lipid Res., 46, 1053-1060 (2005). (3) J. W. Suttie, Vitamin K and human nutrition,]. Am. Diet. Assoc., 92, 585-590 (1992). (4) G. G. Dolnikowski, Z. Sun, M.A. Grusak, J. W. Peterson, and S. L. Booth, HPLC and GC/MS
DETERMINATION OF VITAMINS K 1 AND K3 IN COSMETICS 467 determination of deuterated vitamin K (phylloquinone) in human serum after ingestion of deuterium labeled broccoli,]. Nutr. Biochem., 13, 168-174 (2002). (5) M. Reta, M. E. Figueira, H. M. Felipe, and C. M. M. Almeida, Analysis of vitamin K in green tea leaves and infusions by SPME-GC-FID, Food Chem., 100, 405-411 (2007). (6) S. Odes and 0. Cagindi, Determination of vitamin K 1 content in olive oil, chard and human plasma by RP-HPLC method with UV-Vis detection, Food Chem., 100, 1220-1222 (2007). (7) V. I. Piironen, T. J. Koivu, 0. Tammisalo, and P. Mattila, Determination of phylloquinone in oils, margarines and butter by high-performance liquid chromatography with electrochemical detection, Food Chem., 59, 473-480 (1997). (8) T. J. Koivu-Tikkanen, V. Ollilainen, and V. I. Piironen, Determination of phylloquinone and mena quinones in animal products with fluorescence detection after postcolumn reduction with metallic zinc,]. Agric. Food Chem., 48, 6325-6331 (2000). (9) G. W. Chase, Jr., R.R. Eitenmiller, and A. R. Long, The liquid chromatographic analysis of vitamin K 1 in soy based infant formula using matrix solid phase dispersion,]. Liq. Chromatogr. Rel. Techn., 23, 423-432 (2000). (10) Y. Suhara, M. Kamao, N. Tsugawa, and T. Okano, Method for the determination of vitamin K homologues in human plasma using high-performance liquid chromatography-tandem mass spec trometry, Anal. Chem., 77, 757-763 (2005).
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