CASSIA HPTC IN CONDITIONING SHAMPOO 647 80 97 0 100 200 265 293 321 341 300 m/z Hair treated with Clarifying Shampoo Hair treated with Shampoo containing Quaternized Cassia 381 425 400 500 Figure 6. Representative negative-ion ToF-SIMS spectra of hair treated with clarifying shampoo (top) and the cassia formula (bottom). Table III Secondary Ions Identified from Cassia HPTC, Hair Surface, and Anionic Surfactants Source Cassia HPTC Hair surface Alkyl sulfates (AS) Alkyl ether sulfates (AES) Mass/charge ratio 45 58 100 102 132 134 26 42 341 265 293 321 309 337 353 365 381 397 409 425 Secondary ions C4H50 + C3H8N+ CsHsO2 + CsH 1 oO2 + CsHsO4 + CsH10O4+ CN- (from protein) CNO- (from protein) C20H4 1Cos- (from 18-MEA) C12H25SO4- (Cl2AS) C 14 H29SO4- (C14AS) C l( ,H33SO4- (C16AS) C 14 H29SO5- (C12AE1S) C 1 6H33SO5- (C14AE1S) C 1 6H35SO6- (C12AE2S) C 18 H37SO5 - (C16AE1S) C 18 R37SO6- (C14AE2S) C18H37SO7 (Cl2AE3S) C20H41SO6- (Cl6AE2S) C20H41 SO7 - (Cl4AE3S) The spectroscopy measurements were complemented with imaging analyses to deter­ mine the homogeneity of the polymer film coverage on the hair surface. Figure 7 shows representative secondary-ion images from hair treated with clarifying shampoo (top) and hair treated with cassia formula (bottom). The total-ion image is also shown to indicate

648 JOURNAL OF COSMETIC SCIENCE Hair treated with Clarifying Shampoo Cassia Anionic Surfactant Hair Surface Protein 18-MEA Total Jon Hair Treated with Cassia Formula Cassia Anionic Surfactant Hair Surface Protein 18-MEA Total Jon Figure 7. Secondary-ion images of hair treated with clarifying shampoo (top) and with the cassia formula (bottom). The image field of view is 100 µm x 100 µm. The images are the sum of the respective secondary-ion images for the secondary ions listed in Table III. After the summation, the square root of each intensity image was taken to improve image contrast. the location of the hair fiber in the image. The cassia HPTC image for the hair washed with clarifying shampoo indicates very low background signaling at these masses. This background signal does not indicate that cassia HPTC is present on the hair treated with clarifying shampoo, but rather that other hair surface components also generate a low