280 JOURNAL OF THE SOCIETY OF COSMETIC CHEMISTS I 2 3 4 5 PPD + OHP: • •,• I 2 3 4 5 Figure 5. Hair coloring effect by UOD and TYR. Numbers 1-5 show the enzyme solutions containing different enzyme activities as follows: (1) 500 U TYR, (2) 1,000 U TYR, (3) 5,000 U TYR, (4) 10,000 U TYR, (5) 2,000 U UOD. Table III Effect of Dye Solutions Containing UOD or TYR on Hair Lightness Lightness Enzyme Enzyme activity (IU) PPD PPD + OHP TYR 500 7-7.5 6-6.5 TYR 1,000 6.5-7 6-6.5 TYR 5,000 6 5.5-6 TYR 10,000 6 5.5 UOD 2,000 4 4.5-5 by the replacement of oxygen in the formulation with nitrogen. For permanent waving lotions, an oxidative agent, sodium bromide, in the neutralizing lotion causes the hair stiffness. However, enzymatic neutralization makes it possible to get rather softer waving than from existing products.

HAIR COLORING AND WAVING 281 SDS Treated Figure 6. Hair waving experiment: (1) NaBrO3, (2) UOD, (3) no neutralizer.