323 Microsponge Loaded Topical Gel model terms are not significant. If there are many insignificant model terms (not counting those required to support hierarchy), model reduction may improve your model. Scanning electron microscopy. Scanning electron microscopy of microsponge is shown in Figure 5. The shape of the microsponge was found to be spherical, and the size of the microsponge was in the micrometer range. Moreover, the micrograph also revealed the same agglomeration of microsponges, which might be due to the evaporation of water present in the formulation during sample preparation prior to SEM analysis (22). Figure 3. 3D graphic representation of in vitro drug release %dependence from studied variables. Table V Response 2: Entrapment Efficiency Source Sum of squares df Mean square F value p value Model 2,385.39 9 265.04 4.40 0.0206 A-PLGA 21.84 1 21.84 4.19 0.0794 B-span 80 318.77 1 318.77 4.89 0.0164 C-PVA 204.03 1 204.03 4.85 0.0245 AB 342.70 1 342.70 3.97 0.0152 AC 0.0072 1 0.0072 4.01 0.0497 BC 323.60 1 323.60 4.93 0.0162 A2 508.83 1 508.83 4.61 0.0983 B2 588.53 1 588.53 5.33 0.0821 C2 259.25 1 259.25 4.35 0.0200 Residual 441.46 4 110.36 Cor Total 2,826.84 13 AB: PLGA &SPAN 80 AC: PLGA &PVA BC: SPAN 80 &PVA df: degrees of freedom PLGA: poly lactic-co-glycolic acid PVA: polyvinyl alcohol.

324 JOURNAL OF COSMETIC SCIENCE In vitro drug release studies for microsponges. In vitro drug release for the developed microsponge formulation was carried out by Franz diffusion cell method. The release was found to be 52.42 ± 0.72 in 8 hours. FORMULATION AND EVALUATION OF GEL CONTAINING DRUG-LOADED MICROSPONGES Determination of pH. The pH of the developed gel (F1) was found to be 5.82 ± 0.18. Determination of spreadability. Bioavailability and therapeutic property of the topical formulation depends upon the spreadability. The spreadability is expressed of time in Figure 4. 3D graphic representation of entrapment efficiency %dependence from studied variables. Figure 5. Scanning electron microscopy.