JOURNAL OF THE SOCIETY OF COSMETIC CHEMISTS cedure stimulated a copious flow of saliva which functioned both as a solvent for the chlorophyllins and as a buffer of gastric acidity. Results of experimental studies which will be reported elsewhere have shown that, by virtue of its stimulant effects upon the secretion of saliva, administration of the chlorophyllins incorporated in lozenges obviates precipitation of the chlorophyll de- rivatives in the stomach (29). Although neither samples of urine nor samples of perspiration were collected on the first three days of the second control period, the in- gestion of asparagus was continued first control period, the test period, and the last three days of the second control period. From the conforma- tions of the sections of the curve depicting average changes in per- spiratory odors, it is evident the daily administration of 45 mg. of chlorophyllins "a" and "b" during five consecutive days had no signif- icant effects upon the capacities of perspiration to develop odors during storage for twenty-four hours at 37øC. The conclusion stated in the preceding paragraph has been based upon data presented in the following tabulation. TABULATION C--COMPaRATIVE MEAN II•TENSlTIES OF ODORS DETERMINED II• SAMPLES OF PEKSPIKATION COLLECTED FKOM FIFTEEN SUBJECTS DURING CONtTKOL AND TEST PEKIODS Intensities of Odors After Incu- bation at 37øC. for 24 Hours as Dilutions with Air Number of Sam- ples of Perspira- Standard Period of Experiment tion Analyzed Mean Error of Mean First control 75 50 0.62 Test 75 57 0.13 Second control 30 52 0.40 on these days, as well as, upon the last three days of this period. On all days of the first control and test periods and on the last three days of the second control period, the sweating tests were carried out during the hour preceding the con- sumption of asparagus. Chart VII presents summaries of results of determina, tions, by the air- dilution method, of the odor-pro- duction potentialities of perspira- tion and of the intensities of odors in the p.m. samples of urines as averages for 15 subjects during the In contrast with the results of determinations of perspiratory odors the sections of the curve for average results of tests of samples of urine during the period of administration of the chlorophyllins does indicate uniform decreases in intensities of odors below the corresponding levels of both the first and second control periods. The significances of these decreases in intensities in odors of urine which are attributable to the administration of the chlorophyllins daily over periods of five consecu- tive days become evident upon

TESTING DEODORANTS WITH CHLOROPHYLL AND DERIVATIVES 63 review of the data presented in wide margins, the intensities of the subsequent tabulation. odors in the same samples as deter- TABULATION D--CoMPARATIVE MEAN INTENSITIES OF ODORS DETERMINED IN SAMPLES OF UeaNE COLLECTED FROM FIFTEEN SUBJECTS DURIN• CONTROl- AND T•sT PERIODS Period of Experiment Intensities of Odors of Samples of Urine Collected During 7 Hours After Asparagus as Dilutions with Air Number of P.M. , -, Samples of Urine Standard Analyzed Mean Error of Mean First control 75 20 0.88 Test 75 5 0.51 Second control 30 222 0.66 The predominant odor which was demonstrable in the volumes of air drawn through the samples of urine was that of methyl mercaptan which is volatilized readily by aeration of the samples of urine. In order to ascertain the effects of the oral ingestion of chlorophyllins upon the less readily volatilized sources of odors, other than methyl mercaptan, determinations of odors in urine were carried out by the water- dilution method. All samples of urine were heated on a water-bath to 40øC. before undertaking these tests and, also, all dilutions were made with water previously warmed to 40øC. Results of evaluations of intensi- ties of odors by water dilutions of urine are summarized in Chart VIII. Comparisons of the values for the p.m. samples plotted in this chart with the air-dilutions for aliquots of the same samples in Chart VII show that the dilutions with water requisite to render odors of the p.m. samples of urine, collected during the first control period, imperceptible exceed, by mined by the air-dilution method. Differences between these two sets of values for any one sample are representative of odors from sources which are not volatilized by aera- tion but are vaporized under condi- tions of the water-dilution pro- cedure. Qualitative characteristics of the odors from the warmed samples diluted with warm water indicated definitely that a variety of components of urine contributed to the sum of olfactory stimuli as evaluated through an osmoscope. However, the finding of greatest practical significance is the fact that, during the period of oral administration of the chlorophyllins, the sums of odors from all contribut- ing sources in the urine were re- duced to levels at which they were perceptible only in the undiluted samples. This statement is appli- cable to the a.m. as well as to the p.m. samples of urine. Curves in both Charts VII and VIII indicate rises in intensities of odors during the last three days of the second control period. How- ever, both methods of evaluating