PROTEC T1VE Efl◄ECT ◄ OF P AEONll◄LORlN 3 Figure 5. 3D images obtained from s ·in replicas of one volunteer. (.A,C) "before treatment." (B,D) "after treatment." (A,B) sample treatment. (C,D) placebo treatment. topically uppliecl anti-o. idants could prevent the symptoms of ski11 aging, especially the fi rnrntion , , f wrink l ·s O ) ... rarting from rh . _ id as, we developed a new cosmetic ,1Cliv in rrcdicnr, pnnially p 1rificd paeoniflorn. .1t was primarily tL rgeted to preve11t cdlular senc. cence bernus. of i s inhibitory acti ity on J3-galactosidast:, but we mainly f cus, d o irs protective,,. ntioxidacive properties. :c far, w� hav� reported the protective eHecrs f PF on 0 iclativ D1 TA damage and the anti-wrinkle efect, f hut the exact me hanism by ,vhich PP ex .rts its effects stiU remains to be elucidated. CONCUJ"JON In this study, we have developed a new cosmetic it gredient, parcial.l y purified paeoni- florin (PF), from the root of Paeoniae lactiflm·.,J which has lon.g been u •,�d as a Chin se medicinal herb. We evaluated che protective effe t of PF, and om resuhs clearly show that the p.artiall purified paeoniflorin, with its potential anti-oxidative properties, prevented DVB-induced DNA damage both in cul ured human keratinocytes and hair- less mouse skin. Furthermore, when a cosmetic pr paration containing 0.5% PF was topicaUy applied to human skin for eight \-..' .k , chcrc \1.ras a srariscically significant decre��e in facial \;\'tinkle . Our dma suggest tha par ially purified pa oniflorin could be used a ,1 cosmetic ingredienr for general anti-aging purposes or for reduct.ion in ,vrinklc R Eir◄ER El TCE. .. (1) L. H. Kligman, Photo�ging: 1-hnifestations, prevention, and treatment, D 'l'tJMtol. Clin., ·, 517-528 (1986).

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