272 JOURNAL OF COSMETIC SCIENCE Figure 6. Hair fiber pretreated with NaCI (2 min), dried, and then immersed in silver nitrate (2 rain) followed by exposure to light. Deposits of silver are present within the cuticle cmc and progress into the a-layer and exocuticle (•-•,•). Distinct silver deposits surround each macrofibril within the cortex ((D). Scale bar = 0.25 pm. 5(ii). For hair immersed in sodium chloride (60 minutes) followed by immersion in aqueous silver nitrate (60 minutes), and then exposed to light, extensive deposits of silver were evident along the cuticle cmc's of all cuticle cells (Figures 8, 9). Cascade-like deposits of silver, apparently originating in the cmc were seen

PENETRATION INTO HAIR FIBER 273 Figure 7. Hair fiber pretreated with NaCI (2 rain), dried, and then immersed in silver nitrate (2 rain) followed by exposure to light. Cascade-like silver deposits (4-,,•) adjacent to the outer beta layer of the cuticle cell membrane complex are associated with distinct silver grains within the crnc. Scale bar = 0.5 !•m. throughout the a-layer and exocuticle. Deposits of larger particle sizes were also evident in the endocuticle. In the cortex the inter-macrofibrillar material was well marked by the presence of silver granule deposits (Figure 10). In low-power micrographs this component could be traced throughout the cortex by the pres-

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