JOURNAL OF COSMETIC SCIENCE 218 (D) SUBSTANTIVITY (ANIONIC SURFACTANT SYSTEM) PURPOSE Determine the substantivity of quaternium compounds in an anionic surfactant delivery system to human hair. PROCEDURE – STM – PE#4 Quat solutions: Titrated quat solutions INCI: Polyquaternium-10 Surfactants: Sodium lauryl sulfate Sodium laureth sulfate Lot # Name ARL-4-86A ARL-4-86B ARL-4-86C ARL-4-86D 565720 Polyquaternium 10 0.5% 0.5% — — 0321605 Sodium lauryl sulfate 40.00% — 40.00% — 04107056 Sodium laureth sulfate — 40.00% — 40.00% 062906 Deionized water 59.50% 59.50% 60.00% 60.00% Table IX Cationic Substantivity: 0.5% Quat Solutions Result Quat solution Tress sample Treatment Positive Negative AMB A ARL-4-85A Pink — AME B ARL-4-85B Purple — AMG C ARL-4-85C — No color change AMM D ARL-4-85D Purple — AEB E ARL-4-85E Purple — AEG F ARL-4-85F — No color change CaMB G ARL-4-85G Purple — CaMG H ARL-4-85H No color change DMB I ARL-4-85I Purple — DMG J ARL-4-85J Purple — DMM K ARL-4-85K Purple — MMB L ARL-4-85L Purple — MMG M ARL-4-85M Purple — MMM N ARL-4-85N Purple — — O Positive control Pink — — P Negative control — No color change

2010 TRI/PRINCETON CONFERENCE 219 Table X Substantivity of Titrated Quat Solutions in SLS Result Tress sample Treatment Positive Negative A Test — No color change B Test — No color change C Test — No color change D Test — No color change E Test — No color change F Test — No color change G Test — No color change H Test — No color change I Test — No color change J Test — No color change K Test — No color change L Test — No color change M Test — No color change N Test — No color change O Positive control — No color change P Negative control — No color change Table XI Substantivity of Titrated Quat Solutions in SLES Result Tress sample Treatment Positive Negative A Test — No color change B Test — No color change C Test — No color change D Test — No color change E Test — No color change F Test — No color change G Test — No color change H Test — No color change I Test — No color change J Test — No color change K Test — No color change L Test — No color change M Test — No color change N Test — No color change O Positive control — No color change P Negative control — No color change