JOURNAL OF COSMETIC SCIENCE 212 Preparation of 10% active quat: Ingredient w/w Stock quat solution (35% w/w) 28.50% Distilled water 71.50% Combine ingredients listed with slow to medium agitation. Mix at 20°-25°C until uni- form. Then, (1) Using a hot plate and stir bar set on medium agitation, slowly titrate surfactant test solution with quat solution using a 5-ml disposable pipette, at a rate of one drop per second. Continue adding quat solution until a precipitate is observed (subjective evaluation). (2) The solution will appear cloudy and translucent at this point. Record the amount (grams) of quat solution added at the cloud point, fi nal pH, and the viscosity. Repeat for remaining quat solutions. Store at 20°-25°C. Observe solutions after 24 hours. If solution remains cloudy, then titration is complete. If solution is clear, then repeat step 2. Perform titration for each quat using SLS and SLES individually. Titration data is shown in Tables II–V. Calculations grams of quat added %Quat added (50 grams of quat added) = ´100 + Table II Titration Data (SLS) Quat Sample Amount of quat solution added to achieve haze point in SLS (g) Final pH Viscosity (cps) Notes AMB 9.75 7.6 4,400 Formed an opaque, pearlescent gel beyond the haze point AME 6.28 7.1 10 Did not form a gel AMG 30.49 8.1 10 Did not form a gel AMM 17.63 7.8 10 Did not form a gel AEB 14.58 7.9 10 Did not form a gel AEG 29.53 7.7 10 Did not form a gel CaMB 25.72 7.6 1,000 Formed a gel CaMG 44.47 7.1 1,000 Formed a gel DMB 18.33 7.6 10 Did not form a gel DMG 40.25 7.6 12,000 Formed a gel DMM 23.85 7.6 6,000 Formed a gel MMB 15.28 7.4 14,000 Formed a gel MMG 31.02 8.0 13,000 Formed a gel MMM 21.25 8.0 13,400 Formed a gel

2010 TRI/PRINCETON CONFERENCE 213 Table III Titration Data (SLS) Quat Sample Amount of quat solution added to achieve haze point in SLS (g) Viscosity (cps) Notes AMB 9.75 10 Did not form a gel AME 6.28 10 Did not form a gel AEB 14.58 10 Did not form a gel AMM 17.63 10 Did not form a gel DMB 18.33 10 Did not form a gel AEG 29.53 10 Did not form a gel AMG 30.49 10 Did not form a gel Soft Quats Quat Sample Amount of quat solution added to achieve haze point in SLS (g) Viscosity (cps) Notes MMB 15.28 14,000 Formed a gel MMM 21.25 13,400 Formed a gel DMM 23.85 6,000 Formed a gel CaMB 25.72 1,000 Formed a gel MMG 31.02 19,200 Formed a gel DMG 40.25 12,000 Formed a gel Table IV Titration Data (SLES) Quat Sample Amount of quat solution added to achieve haze point in SLS (g) Final pH Viscosity (cps) Notes AMB 18.67 6.6 10 Did not form a gel AME 4.47 7.0 10 Formed a gel AMG 25.04 7.2 1,000 Formed a gel AMM 17.44 7.2 10 Did not form a gel AEB 18.35 7.2 10 Did not form a gel AEG 38.72 7.1 1,000 Formed a gel CaMB 24.31 7.6 1,000 Formed a gel CaMG 46.23 7.3 1,000 Formed a gel DMB 11.09 7.3 10 Did not form a gel DMG 28.37 7.9 6,800 Formed a gel DMM 20.00 7.0 6,200 Formed a gel MMB 25.00 7.1 10 Formed a gel MMG 26.68 7.1 40,000 Formed a gel MMM 20.23 7.3 50,000 Formed a gel