dO(X) 3•0 :)2'00 2KO 2aO0 2O00 ISO0 l•)OO 1,400 1200 mOO eOO I J I -•o 7o Figure 9. Internal reflection infrared spectra profiling human skin in sttu, as epidermal layers were success- fully stripped away. (•) Forearm skin of male volunteer, following 2 groups of 10 "Scotch tape strippings" of epidermal layers. Compare with less damaged skin of same subject in Figure 8. • After third group of 10 skin strippings. ¸ After fourth group of 10 skin strippings. Note that N-H absorption band is almost obscured by increasing O-H band intensity. (•) After fifth group of 10 skin strippings. (•) After sixth group of 10 skin strippings, producing a "glistening" wound of dimensions 80 mm x 60 mm. (•) Healed skin, 6 days following wound production by 60 "scotch tape strippings." 300

z Figure 10. Internal reflection infrared spectra ofexudates from skin wounded to successively deeper layers. (•) Exudate upon contact with skin "stripped" 20 times with Scotch tape. Compare with skin spectrum, in situ, in Figure 9. (• Exudate after 30 strippings of epidermal layers. ¸ Exudate after 40 strippings. Exudate after 50 skin strippings. Note the absence of lipid absorption bands from this spectrum of purely proteinaceous matter. (•) Exudate from "glistening" forearm wound produced by 60 skin strippings. (•) Exudate from wounded skin area after 6 days of healing. 301