589 Specificities of Microbiota From Sensitive Skin
prevalence of greater than 30% for either cohort. Considering genera having a prevalence
higher than 50% for either cohort (NS or SS), we observed that the genus Cutibacterium was
found in all the volunteers regardless of cohort. The genus Staphylococcus was also found
in all NS volunteer, but the prevalence slightly decreased by 3% in SS. The prevalence
was decreased by 23% in the Streptococcus genus, by 19% in the Anaerococcus, and by 9%
in the Paracoccus and Bradyrhizobium. On the contrary, we observed an increase in SS of
the prevalence in Corynebacterium genus by 7%, Acinetobacter by 47%, Kocuria by 45%, and
Enhydrobacter by 65%.
Next, we compared the two cohorts to examine the presence of common genera and to
determine variations in abundance (Figure 3). Cutibacterium and Staphylococcus are the two
most abundant genera, but the proportions were reversed between the two cohorts. NS
showed 47% Cutibacterium abundance and 39% Staphylococcus, compared to the SS with
60% Cutibacterium, and only 22 %Staphylococcus. Ruminococcus, Anaerococcus, and Romboutsia
were present in the top 10 of most abundant genera in NS, but were absent from this
ranking in SS. On the contrary, Bacillus, Acinetobacter, Actinomyces, Kocuria, and Micrococcus
were more abundant in SS when compared to NS.
When looking at the species level Cutibacterium genus, the more prevalent and abundant
genus in both cohorts, and found increased in SS by 1.28-fold (from 47% to 60%, Figure 3),
we observed that, in NS the most prevalent and abundant members of the Cutibacterium
were 4 species: Cutibacterium acnes, Cutibacterium granulosum, Cutibacterium namnetense and
Cutibacterium avidum with a respective relative abundances of 46%, 2%, 0.4%, and 0.1%
(data not shown). In SS subjects, only 3 species of Cutibacterium were recovered, with
Cutibacterium avidum no longer being detected. In addition, Cutibacterium acnes was increased
Figure 2. Sequencing analysis between normal and sensitive skin. Illustration of selected 20 genera having
more than 30% prevalence in either cohort (percentage of negative or positive variation in SS versus NS are
given for species having a prevalence greater than 50% for either cohort).

590 JOURNAL OF COSMETIC SCIENCE
in SS reaching an abundance of 60% (increase by 1.28-fold) while Cutibacterium granulosum
abundance was decreased to 0.67% (decrease by 3-fold).
In the case of Staphylococcus genus, the second most abundant genus and found decreased
by 1.75-fold in the SS (from 39% to 22%, Figure 3), we observed at the species level that
the S. epidermidis and S. capitis were the 2 most abundant Staphylococcus species and that
both were decreased in SS (approximately by 1.6-fold). We also observed a decrease of
Staphylococcus hominis and S. caprae in SS (respectively by 1.5 and 1.8-fold, Figure 4), whereas
Staphylococcus equorum and Staphylococcus saprophyiticus almost disappeared in SS. Finally,
47
60
39
22
0%
10%
20%
30%
40%
50%
60%
70%
80%
90%
100%
Normal skin Sensitive skin
Cutibacterium
Staphylococcus
Corynebacterium
Streptococcus
Lautropia
Ruminococcus
Anaerococcus
Paracoccus
Romboutsia
Bradyrhizobium
Bacillus
Acinetobacter
Actinomyces
Kocuria
Micrococcus
Figure 3. Sequencing analysis between normal and sensitive skin. Illustration of the 10 most abundant
genera present in each cohort.
19
11
13
8
9
5
3
3
3
2
0
10
20
30
40
50
60
Normal skin Sensitive skin
S.epidermidis
S.capitis
S.equorum
S.hominis
S.saprophyticus
S.caprae
S.cohnii
S.pasteuri
S.haemolyticus
S.auricularis
S.aureus
S.saccharolyticus
S.petrasii
S.lugdunensis
S.warneri
S.xylosus
S.pettenkoferi
Figure 4. Sequencing analysis between normal and sensitive skin. Illustration of Staphylococcus species
abundance relative to the global abundance bacterial species between normal and sensitive skin.
Abundance
(%)
Staphylococcus
abundance
(%)