578 JOURNAL OF COSMETIC SCIENCE
ABBREVIATIONS
3D: Three-dimensional
BHI: Brain heart infusion
CoNS: Coagulase negative Staphylococci
CV: Coefficient of variation
CFU: Colony forming units
INCI: International Nomenclature of Cosmetic Ingredients
LR: Logarithmic reduction
MALDI-TOF: Matrix-assisted laser desorption ionization -time of flight
MIC: Minimum inhibitory concentration
RHE: Reconstructed human epidermis
TSB: Tryptone Soy Broth
UV: Ultraviolet radiation
ACKNOWLEDGMENT
GLYcoDiag acknowledges Mateja Senicar (PhD) and Yasmina Traoré (MA) for their
contribution during the early stages of development of this in vitro coculture model.
CONFLICTS OF INTEREST
Sophie Cambos, Christine Garcia, Alicia Roso, and Sophie Pécastaings are employed by
the company Seppic. Laura Bauchet and Benoît Roubinet are employed by GLYcoDiag.
Richard Martin is a consultant of Mercurialis Biotech in the field of microbiology and was
paid by Seppic. Seppic cofounded the research with GLYcoDiag.
REFERENCES
(1) Byrd AL, Belkaid Y, Segre JA. The human skin microbiome. Nat Rev Microbiol. 2018 16(3):143–155.
doi:10.1038/nrmicro.2017.157
(2) Zhu Y, Yu X, Cheng G. Human skin bacterial microbiota homeostasis: A delicate balance between
health and disease. mLife. 2023 2(2):107–120. doi:10.1002/mlf2.12064
(3) Swaney MH, Nelsen A, Sandstrom S, Kalan LR. Sweat and Sebum Preferences of the Human Skin
Microbiota. Microbiol Spectr, Claesen J, ed. 2023 11(1):e04180–22. doi:10.1128/spectrum.04180-22
(4) Jensen MG, Svraka L, Baez E, Lund M, Poehlein A, Brüggemann H. Species- and strain-level diversity
of Corynebacteria isolated from human facial skin. BMC Microbiol. 2023 23(1):366. doi:10.1186/
s12866-023-03129-9
(5) O’Sullivan JN, Rea MC, O’Connor PM, Hill C, Ross RP. Human skin microbiota is a rich source
of bacteriocin-producing staphylococci that kill human pathogens. FEMS Microbiol Ecol. 2019 95(2).
doi:10.1093/femsec/fiy241
(6) Lee H, Lee DG, Jo H, et al. Comparative whole genome analysis of face-derived Streptococcus infantis
CX-4 unravels the functions related to skin barrier. Genes Genomics. 2024 46(4):499–510. doi:10.1007/
s13258-024-01495-w
ABBREVIATIONS
3D: Three-dimensional
BHI: Brain heart infusion
CoNS: Coagulase negative Staphylococci
CV: Coefficient of variation
CFU: Colony forming units
INCI: International Nomenclature of Cosmetic Ingredients
LR: Logarithmic reduction
MALDI-TOF: Matrix-assisted laser desorption ionization -time of flight
MIC: Minimum inhibitory concentration
RHE: Reconstructed human epidermis
TSB: Tryptone Soy Broth
UV: Ultraviolet radiation
ACKNOWLEDGMENT
GLYcoDiag acknowledges Mateja Senicar (PhD) and Yasmina Traoré (MA) for their
contribution during the early stages of development of this in vitro coculture model.
CONFLICTS OF INTEREST
Sophie Cambos, Christine Garcia, Alicia Roso, and Sophie Pécastaings are employed by
the company Seppic. Laura Bauchet and Benoît Roubinet are employed by GLYcoDiag.
Richard Martin is a consultant of Mercurialis Biotech in the field of microbiology and was
paid by Seppic. Seppic cofounded the research with GLYcoDiag.
REFERENCES
(1) Byrd AL, Belkaid Y, Segre JA. The human skin microbiome. Nat Rev Microbiol. 2018 16(3):143–155.
doi:10.1038/nrmicro.2017.157
(2) Zhu Y, Yu X, Cheng G. Human skin bacterial microbiota homeostasis: A delicate balance between
health and disease. mLife. 2023 2(2):107–120. doi:10.1002/mlf2.12064
(3) Swaney MH, Nelsen A, Sandstrom S, Kalan LR. Sweat and Sebum Preferences of the Human Skin
Microbiota. Microbiol Spectr, Claesen J, ed. 2023 11(1):e04180–22. doi:10.1128/spectrum.04180-22
(4) Jensen MG, Svraka L, Baez E, Lund M, Poehlein A, Brüggemann H. Species- and strain-level diversity
of Corynebacteria isolated from human facial skin. BMC Microbiol. 2023 23(1):366. doi:10.1186/
s12866-023-03129-9
(5) O’Sullivan JN, Rea MC, O’Connor PM, Hill C, Ross RP. Human skin microbiota is a rich source
of bacteriocin-producing staphylococci that kill human pathogens. FEMS Microbiol Ecol. 2019 95(2).
doi:10.1093/femsec/fiy241
(6) Lee H, Lee DG, Jo H, et al. Comparative whole genome analysis of face-derived Streptococcus infantis
CX-4 unravels the functions related to skin barrier. Genes Genomics. 2024 46(4):499–510. doi:10.1007/
s13258-024-01495-w











































































































































