(a) '50 1C!l 1!50 :;:,DO 250 /llO (b) (c) � I·UALIZATION OF ·.,Kf BARRIER PERTURBATIO (d), 100 1!.o m 2-50 1,!() ·1� :aw 251! !Sil l!!O 11(1 , - (e) ·� 100 l!D 16 (f)1 14 12 10 101J ,so � � 1110 150 200 .l5D figui: �.. Dual- h1mo, I TPM kin imas of· -FTS a z = µm fou· w 2 1 i40 120 ,co !II eo 411 21) m 2!10 16 14 12 10 Cl): (a)-SRB fluorescence (red channel). (c}-Skin autofluoresc nee rh the linear fhmr · cen i y r.lecons · regions m i:ked b rhe yello ·rn1 •· ·, · lmn s in [ s m: [he mor . RB fluorescen e and skin aui:ofluore . Pan d, f, an· th� • nalogous figure$ for p-FT sampi�s (!xposed to soiutio . r scale har in n in rens in RB in en icy for rh el in1;:1 a. ;;ind an i · kin autofluorescence intensiry fur the gree.11-charme arcs ca C:he color [ mg � a low luc do t: o zc:to bJm ·) to a h" h . aim· rc:d/g · ·: C--:01:neocyt:t!'j. white Line in p 5 µm. r · tl corresr ondi · · flu e iffi r nee b · w b · skin autofluor ce t it k • idths. pr, uo - induced RB l etradon into the corneo yte of fo or peak h .ights corr, ponding to the re -chann l e · er · , ·, eak heighc corresponding t,o the gr en-channel specn · , , red a · nes jn Fig11H-e b). Thi.:s. · mvides e idence chat D promote· RB ,etrati. 1e lip"d bjfa. er in ·ddi1:ion rn prom tin,g pe.netracion into th comeocyces. Th r lt. ind·­ car chat not only do s SOS rupture the comeocyte envelopes and denatuJ:1e the corneo-

2 2 JO RNAL OF MET'1C CI.ENCE (a) 9) 100 1!'.;l :m (b) .. I 100 150 ,!lj (c) igur 6,, hanr:ieoi TP1r kin ima�t:-s of p 9 nt z = µm that were (c'"Xposecl to Soluri.oil v 00 we gl ): (a)--SRB fluo ceo ai in moflu nc (gr n dwnn I) ·t1 he linear fluoresc nee inrensity dec.11s:truct1011 re. •ion mark d by che yellow rectan •l .. The r d and the gr en line in rt b rl!prest!m norm. li�ed . SR.B fiuon:sc::tnce nd "k·n 1u1 ,oJl.uoresc:tnce spcc:t 1, n:s_pt�ri tl)•. The c ior .1 b r. i ndi care an ioc ren. e in · RB i ni: nsi ry for rhe red- hatmel i m e ( ) anl an increas in kin autofluorescenGf intensity for the grnfo chann ·l image (c), as th.e coJor progrl"s l"S from a low , lue d.o ·e to zt.ro, black) to a] igb Lue (red/.gr n). K, y: C--or11u:ocyre l ·c Lin in _re a - 25 µm. cyte keratin·, but that it also disrupts the intercellular lipid bifo)ers, ther,eby inducing RB penetration. into 11:he cornen ,,res (the brick) a.nd into the lipid bilayer (the mortar) in the C