280 RNAL Of , 'OSMETIC SCIEN E 1 lOO (a.) 12!111 (d) 1100 00 1000 t00 100 9:,) 100 1$0 ,e;:o SIii 200 ◄OI! JOO 2SO 511, 100 1� w::l 2511 ,(e) (c) � 11111 Figllllre Dual-chann 1 TPM skin images of p-FTS samples at z = 3 µm that rere ex1 os,ed co solution i (1 wt% D ): f!}-S B fluor .cc·ncc (red channel). (c)-Skin autofluorescrnce (green channel.·, with the linear fluores,cenic incensity de onsrruc1rion re, ions markeJ by th yellow rectangles. The , d and rhe •r.:en lines in part b r•t'pres!nt the normalized RB fluorescence and skin autofluorescence spe-ctro.) respecti eJ . P res d, f, ml e ar the an log m figure r p-F , samples tl flt were exposed to sofotion ii (1 wt% SD + 10 , t% glycerol). The color scale ha.rs indicate an increase in SRB intem,ity fur the red-chann 1 jm ge parts a aucl d) and n increase in skjn autufluores .ence intensity for the green-channd images (parts c and f) as th color ! mgr · from a I w value cl · o zero (bin ) to a high v lue (re I/green). y: C---cotrieocyte white line in pact a-25 µm. x = 102 · , and ti · - -- c, ks ar x = 10 pi 16 "' , pix 1 and x = 225 I · - �ls. T ur e, - d o rh posi ion inr, rceHufar lipid bilayer · ns shm1m in th y a · · · n lu 'n the corr ponding n-chann 1 1 s c _ el I P A ·kin image n in , igur also h L •_ l n-chann 1 TPM skin imag,e (s e Fig c). The . o o y · 1 ectrum peak v i ·hs (see he red lin ot chat rhe clisumce b tw n a h o c a�k corres.poo ls at prcn:imaicel co l .e l, ngth o a corneocyte (20 ,0 �.m), thH�by imp.lying that che pcl. irion of these peak denme rhe l io11s of th iiu: r-ceJlular bilay r region i the SC (20-23,46).

(a) '50 1C!l 1!50 :;:,DO 250 /llO (b) (c) � I·UALIZATION OF ·.,Kf BARRIER PERTURBATIO (d), 100 1!.o m 2-50 1,!() ·1� :aw 251! !Sil l!!O 11(1 , - (e) ·� 100 l!D 16 (f)1 14 12 10 101J ,so � � 1110 150 200 .l5D figui: �.. Dual- h1mo, I TPM kin imas of· -FTS a z = µm fou· w 2 1 i40 120 ,co !II eo 411 21) m 2!10 16 14 12 10 Cl): (a)-SRB fluorescence (red channel). (c}-Skin autofluoresc nee rh the linear fhmr · cen i y r.lecons · regions m i:ked b rhe yello ·rn1 •· ·, · lmn s in [ s m: [he mor . RB fluorescen e and skin aui:ofluore . Pan d, f, an· th� • nalogous figure$ for p-FT sampi�s (!xposed to soiutio . r scale har in n in rens in RB in en icy for rh el in1;:1 a. ;;ind an i · kin autofluorescence intensiry fur the gree.11-charme arcs ca C:he color [ mg � a low luc do t: o zc:to bJm ·) to a h" h . aim· rc:d/g · ·: C--:01:neocyt:t!'j. white Line in p 5 µm. r · tl corresr ondi · · flu e iffi r nee b · w b · skin autofluor ce t it k • idths. pr, uo - induced RB l etradon into the corneo yte of fo or peak h .ights corr, ponding to the re -chann l e · er · , ·, eak heighc corresponding t,o the gr en-channel specn · , , red a · nes jn Fig11H-e b). Thi.:s. · mvides e idence chat D promote· RB ,etrati. 1e lip"d bjfa. er in ·ddi1:ion rn prom tin,g pe.netracion into th comeocyces. Th r lt. ind·­ car chat not only do s SOS rupture the comeocyte envelopes and denatuJ:1e the corneo-